Objective:To study the chem. constituents from fresh pine needles of Pinus massoniana.Methods:Silica gel,glucan gel G25,Sephadex LH-20,ODS,MCI,YMC and other column chromatog. were used to isolate and purify the compounds,and the structures of the compounds were identified according to the physicochem. properties and spectral data.Results:Fifteen compounds were isolated from fresh pine needles of Pinus massoniana and identified as isotachioside(1),L-tryptophan(2),(+)-catechin-8-C-β-D-glucopyranoside(3),vanillic acid(4),(-)-epicatechin(5),quercetin-3′-O-β-D-glucopyranoside(6),1-(4-hydroxyphenyl)glycerol-3-O-β-D-glucopyranoside(7),4′-methoxy-kaempferol-3-O-galactopyranoside(8),tamarixin(9),(7R,8S)1-(4-hydroxy-3-methoxyphenyl)-2-[4-(3-α-L-rhamnopyranoxypropyl)-2-methoxyphenoxy]-1,3-propanediol(erythro)(10),(7S,8S)1-(4-hydroxy-3-methoxyphenyl)-2-[4-(3-α-L-rhamnopyranoxypropyl)-2-methoxyphenoxy]-1,3-propanediol(threo)(11),massonianoside A(12),hovetrichoside A(13),4-(4-O-β-D-glucopyranosyl-3-methoxyphenyl)-2-butanone(14),3,4-dimethoxyphenyl-1-O-β-D-apiofuranosyl-(1→6)-β-D-glucopyranoside(15),resp.Conclusion:Among them,compounds 1 to 3,6 to 9,13 to 15 are isolated from Pinus massoniana for the first time.