Objective: To establish a method for the dissolution curve determination of trihexyphenidyl hydrochloride tablets in vitro, and to evaluate the consistency of the dissolution profiles of generic and reference preparations in three different kinds of pH mediums. Methods: The paddle method of dissolution determination method was adopted, the rotation was 75 r/min, and the dissolution media were 500 mL pH 1.2, 4.5, 6.8. The dissolution of trihexyphenidyl hydrochloride tablets was determined by UPLC, the cumulative dissolution was calculated, and the similarity of dissolution profiles were evaluated. Results: In three different pH dissolution media, the cumulative dissolution rate of generic and reference preparations at 15min all reached more than 85%, which was a rapid dissolution It indicated that the dissolution profiles of the generic preparations were similar to the reference ones. Conclusion: The in vitro dissolution behavior of generic tablets is consistent with that of the reference ones.