Hafnium oxide nanoparticles

Results demonstrated favorable safety, injection feasibility, and encouraging oncologic outcomes in patients (n=22) with locally advanced or borderline resectable pancreatic cancer Median Overall Survival of 23 months from date of diagnosis [95% CI; 17 months – not reached] Median Local Progression-Free Survival of 13.3 months from completion of radiation Notable findings observed in exploratory biomarker analyses include: An association between increased circulating tumor mutational burden (cTMB) and LPFS and OS CA19-9 normalization in 59% of patients in the study, and an association between CA19-9 normalization and OS First patient injected in a new cohort that adds standard-of-care concurrent chemotherapy (capecitabine or 5-FU) to radiotherapy-activated JNJ-1900 (NBTXR3) and recruitment is ongoing Investigators concluded that these results support further evaluation in a randomized study An association between increased circulating tumor mutational burden (cTMB) and LPFS and OS CA19-9 normalization in 59% of patients in the study, and an association between CA19-9 normalization and OS Data presented at the 2025 Annual Meeting of the European Society of Radiation Oncology PARIS and CAMBRIDGE, Mass., May 05, 2025 (GLOBE NEWSWIRE) -- NANOBIOTIX (Euronext: NANO –– NASDAQ: NBTX – the ‘‘Company’’), a late-stage clinical biotechnology company pioneering nanotherapeutic approaches to improve treatment outcomes for patients with cancer, today announced the presentation of full results from the completed dose escalation and dose expansion phases of a Phase 1 study evaluating JNJ-1900 (NBTXR3) in patients with locally advanced or borderline resectable pancreatic cancer. The study, conducted by The University of Texas MD Anderson Cancer Center (“MD Anderson”), was presented by principal investigator Dr. Eugene Koay at the 2025 Annual Meeting of the European Society for Radiotherapy and Oncology (ESTRO 2025). Pancreatic ductal adenocarcinoma (PDAC) remains one of the most lethal malignancies, driven by aggressive tumor biology and limited responsiveness to standard therapies. For patients with locally advanced (“LAPC”) or borderline resectable (“BRPC”) disease, the current standard-of-care (“SOC”)—induction chemotherapy followed by chemoradiation—rarely delivers curative outcomes, underscoring the need for novel treatment approaches. “Patients with locally advanced or borderline resectable pancreatic cancer face a particularly urgent unmet need for therapeutic innovation that can provide a meaningful survival benefit with an acceptable safety profile,” said Eugene Koay, MD, PhD, Associate Professor of Radiation Oncology at MD Anderson. “We are encouraged by the results from the completed cohorts and look forward to the continued evaluation of JNJ-1900 (NBTXR3) in combination with standard-of-care chemoradiation after induction chemotherapy.” PRESENTATION #E25-2265: NANORAY Pancreas: A Phase 1 Study of NBTXR3 (JNJ-1900) Activated by Radiotherapy for Locally Advanced or Borderline Resectable Pancreatic Cancer (LAPC or BRPC)Koay EJ, Liu S, Guerrero P, Stokes E, Katz MHG, Ikoma N, Snyder RA, Tzeng CD, Overman MJ, Pant S, Wolff RA, Javle M, Holliday EB, Ludmir EB, Das P, Noticewala S, Koong AC, Tamm EP, Bhutani M This MD Anderson-sponsored Phase 1 study evaluated the potential of radiotherapy(“RT”)-activated JNJ-1900 (NBTXR3) activated by radiation therapy (45 Gy in 15 fractions) to overcome inherent radioresistance in patients with LAPC or BRPC. The majority of patients in the study (20/22) were diagnosed with locally advanced, unresectable disease (LAPC). For clarity, patients with LAPC or BRPC are traditionally treated with induction chemotherapy followed by concurrent chemoradiation. The treatment regimen in the completed dose escalation and dose expansion parts of this Phase 1 study replaced concurrent chemoradiation with RT-activated JNJ-1900 (NBTXR3) after induction chemotherapy. Key Results: Favorable safety profile and injection feasibility were observed (n=22) Median overall survival (“mOS”): 23 months from diagnosis [95% CI; 17 months – not reached] For context, an MD Anderson historical review of 144 patients with LAPC treated at the same center showed a mOS of 19.2 months. Patients in the historical review received induction chemotherapy followed by RT with or without concurrent or maintenance chemotherapy (80% received RT with concurrent chemotherapy) Median local progression-free survival (“mLPFS”): 13.3 months from completion of radiation Two LAPC patients achieved R0 surgical resection For context, an MD Anderson historical review of 144 patients with LAPC treated at the same center showed a mOS of 19.2 months. Patients in the historical review received induction chemotherapy followed by RT with or without concurrent or maintenance chemotherapy (80% received RT with concurrent chemotherapy) Exploratory Biomarker Analyses: Of the 20 patients for whom circulating Tumor Mutational Burden (cTMB) data was available, a notable proportion (40%; 8/20) exhibited increased cTMB, and investigators observed an association between increased cTMB and improved LPFS and OS Normalization of CA19-9, a surrogate for overall survival benefit, was observed in 59% of patients (11/22) and was associated with longer survival in the study. For context, an MD Anderson historical review of 243 patients with LAPC treated at the same center showed normalization of CA19-9 in approximately 17% of patients treated with the standard of care who had elevated CA19-9 levels at diagnosis For context, an MD Anderson historical review of 243 patients with LAPC treated at the same center showed normalization of CA19-9 in approximately 17% of patients treated with the standard of care who had elevated CA19-9 levels at diagnosis Based on the safety and preliminary efficacy findings, investigators concluded that further evaluation of JNJ-1900 (NBTXR3) is warranted in a randomized study. “Our collaboration with MD Anderson has always been driven by a shared commitment to exploring bold new approaches for patients with high unmet need,” said Louis Kayitalire, MD, Chief Medical Officer at Nanobiotix. “Given the extremely poor survival rates in LAPC and BRPC, the results from this Phase 1 study give us confidence in the potential of JNJ-1900 (NBTXR3) to serve as a meaningful addition to the treatment landscape. We are particularly excited about the potential to further enhance outcomes through combination of JNJ-1900 (NBTXR3) with SOC chemoradiation in the study’s new active cohort, and we look forward to advancing this program in pancreatic cancer.” MD Anderson received FDA clearance to expand the study to include a new cohort that combines of JNJ-1900 (NBTXR3) with SOC concurrent chemoradiation after induction chemotherapy. The first patient in the new cohort has been injected, and recruitment is ongoing. Nanobiotix Conference Call Nanobiotix will host a conference call and webcast featuring Nanobiotix Chief Executive Officer and Chairman of the Executive Board, Laurent Levy, to discuss the data on Monday May 5th, 2025, at 8:00 AM EDT / 2:00 PM CEST. Details for the call are as follows: Webcast link: click here Audio-only dial-in link: click here Participants can use the audio-only link above to register and obtain dial-in instructions to listen to the presentation via phone and ask questions during the Q&A session, or participants can use the webcast link to register and listen and watch the slide presentation online; the replay version will be available under the same webcast link shortly after the presentation and will be archived on the Company’s website at www.nanobiotix.com. It is recommended to join 10 minutes prior to the event start. Participants are invited to email their questions in advance to investors@nanobiotix.com. About JNJ-1900 (NBTXR3) JNJ-1900 (NBTXR3) is a novel, potentially first-in-class oncology product composed of functionalized hafnium oxide nanoparticles that is administered via one-time intratumoral injection and activated by radiotherapy. Its proof-of-concept was achieved in soft tissue sarcomas through a successful randomized Phase 2/3 study in 2018. The product candidate’s physical mechanism of action (MoA) is designed to induce significant tumor cell death in the injected tumor when activated by radiotherapy, subsequently triggering adaptive immune response and long-term anti-cancer memory. Given the physical MoA, Nanobiotix believes that JNJ-1900 (NBTXR3) could be scalable across any solid tumor that can be treated with radiotherapy and across any therapeutic combination, particularly immune checkpoint inhibitors. Radiotherapy-activated JNJ-1900 (NBTXR3) is being evaluated across multiple solid tumor indications as a single agent or combination therapy. The program is led by NANORAY-312—a global, randomized Phase 3 study in locally advanced head and neck squamous cell cancers. In February 2020, the United States Food and Drug Administration granted regulatory Fast Track designation for the investigation of JNJ-1900 (NBTXR3) activated by radiation therapy, with or without cetuximab, for the treatment of patients with locally advanced HNSCC who are not eligible for platinum-based chemotherapy—the same population being evaluated in the Phase 3 study. Given the Company’s focus areas, and balanced against the scalable potential of NBTXR3, Nanobiotix has engaged in a collaboration strategy to expand development of the product candidate in parallel with its priority development pathways. Pursuant to this strategy, in 2019 Nanobiotix entered into a broad, comprehensive clinical research collaboration with The University of Texas MD Anderson Cancer Center to sponsor several Phase 1 and Phase 2 studies evaluating JNJ-1900 (NBTXR3) across tumor types and therapeutic combinations. In 2023, Nanobiotix announced a license agreement for the global co-development and commercialization of JNJ-1900 (NBTXR3) with Janssen Pharmaceutica NV, a Johnson & Johnson company. About NANOBIOTIX Nanobiotix is a late-stage clinical biotechnology company pioneering disruptive, physics-based therapeutic approaches to revolutionize treatment outcomes for millions of patients; supported by people committed to making a difference for humanity. The Company’s philosophy is rooted in the concept of pushing past the boundaries of what is known to expand possibilities for human life. Incorporated in 2003, Nanobiotix is headquartered in Paris, France and is listed on Euronext Paris since 2012 and on the Nasdaq Global Select Market in New York City since December 2020. The Company has subsidiaries in Cambridge, Massachusetts (United States) amongst other locations. Nanobiotix is the owner of more than 25 umbrella patents associated with three (3) nanotechnology platforms with applications in 1) oncology; 2) bioavailability and biodistribution; and 3) disorders of the central nervous system. For more information about Nanobiotix, visit us at www.nanobiotix.com or follow us on LinkedIn and Twitter Disclaimer This press release contains “forward-looking” statements within the meaning of the “safe harbor” provisions of the Private Securities Litigation Reform Act of 1995, including, but not limited to, statements regarding the use of proceed therefrom, and the period of time through which the Company’s anticipates its financial resources will be adequate to support operations. Words such as “expects”, “intends”, “can”, “could”, “may”, “might”, “plan”, “potential”, “should” and “will” or the negative of these and similar expressions are intended to identify forward-looking statements. These forward-looking statements which are based on the Company’ management’s current expectations and assumptions and on information currently available to management. These forward-looking statements involve known and unknown risks, uncertainties and other factors that could cause actual results to differ materially from those implied by the forward-looking statements, including risks related to Nanobiotix’s business and financial performance, which include the risk that assumptions underlying the Company’s cash runway projections are not realized. Further information on the risk factors that may affect company business and financial performance is included in Nanobiotix’s Annual Report on Form 20-F filed with the SEC on April 02, 2025 under “Item 3.D. Risk Factors”, in Nanobiotix’s 2024 universal registration document filed with the AMF on April 02, 2025,, and subsequent filings Nanobiotix makes with the SEC from time to time which are available on the SEC’s website at www.sec.gov. The forward-looking statements included in this press release speak only as of the date of this press release, and except as required by law, Nanobiotix assumes no obligation to update these forward-looking statements publicly. Contacts Attachment 2025-05-05 -- NBTX -- Full Ph1 NBTXR3 Results in Pancreatic Cancer -- FINAL

Global development program for JNJ-1900 (NBTXR3) proceeding as planned, with opportunity to address one of the largest untapped markets in oncology through lead programs in head and neck cancer and lung cancer Disciplined financial strategy toward long-term sustainability and growth continues to strengthen financial position through focused allocation of capital, the receipt of a planned milestone payment, the transfer of sponsorship for NANORAY-312, and an amendment to the global licensing agreement for JNJ-1900 (NBTXR3) Curadigm, a next generation nanotherapeutic platform designed to reshape drug design and development across multiple therapeutic classes and disease areas, has launched Cash runway extended into mid-2026 and reduction of operational cash burn expected beyond mid-2026 with €49.7 million in cash and cash equivalents as of December 31, 2024 Clinical data readouts in 2025 from Phase 1 and 2 studies in RM-HNSCC, pancreatic cancer, NSCLC amenable to re-irradiation, melanoma and esophageal cancer Conference call and webcast scheduled for April 3, 2025 at 8:00 A.M. EDT / 2:00 P.M. CEST PARIS and CAMBRIDGE, Mass., April 02, 2025 (GLOBE NEWSWIRE) -- NANOBIOTIX (Euronext: NANO - NASDAQ: NBTX - the “Company”), a late-clinical stage biotechnology company pioneering nanotherapeutic approaches to expand treatment possibilities for patients with cancer and other major diseases, provided an update on operational progress, reported financial results for the year ended December 31, 2024, and announced the filing of its universal registration document (URD) for the financial year ended December 31, 2024 with the French financial market authority (Autorité des marchés financiers or AMF), as well as of the annual report on Form 20-F for the financial year ended December 31, 2024 with the U.S. Securities and Exchange Commission (SEC). Operational Highlights Addressing One of the Largest Untapped Markets in Oncology with JNJ-1900 (NBTXR3)1 Lead programs in head and neck cancer and lung cancer proceeding as planned Ongoing Pivotal Phase 3 LA-HNSCC Study (NANORAY-312) Aligned on intent to transfer the global NANORAY-312 sponsorship from Nanobiotix to Janssen Pharmaceutica NV, a Johnson & Johnson Company (“Johnson & Johnson”) in 2Q2024 to begin preparations for interim analysis. Completed the NANORAY-312 US sponsorship transfer from Nanobiotix to Johnson & Johnson, in 4Q2024, with intent to transfer NANORAY-312 globally to Johnson & Johnson by 3Q2025. Ongoing Randomized Phase 2 Study in Unresectable Stage 3 NSCLC (CONVERGE) First patient dosed in the Johnson & Johnson-sponsored Phase 2 randomized CONVERGE study evaluating JNJ-1900 (NBTXR3) for patients with unresectable stage 3 non-small cell lung cancer (“NSCLC”) in 1Q2025 Multiple early-stage studies across solid tumor types beyond the lead programs continue to progress RM-HNSCC (Phase 1 Study 1100 sponsored by Nanobiotix) Presented new data showing disease control and tumor response in patients treated with JNJ-1900 (NBTXR3) followed by anti-PD-1 as a second-or-later line (2L+) therapy for patients with recurrent or metastatic (“RM”) head and neck squamous cell carcinoma (“HNSCC”) in 2Q2024 Locally Advanced or Borderline Resectable Pancreatic Cancer (Phase 1 Study MDA 2019-1001 sponsored by The University of Texas MD Anderson Cancer Center) Completed a Phase 1 study evaluating JNJ-1900 (NBTXR3) for patients with locally advanced or borderline resectable pancreatic cancer in 4Q2024 and investigators recommended that the encouraging oncologic outcomes coupled with a favorable safety profile warrant further exploration in a randomized study US FDA approved a protocol amendment to the completed Phase 1 pancreatic cancer study in 4Q2024 enabling the launch of a new cohort designed to evaluate JNJ-1900 (NBTXR3) combined with standard-of-care concurrent chemoradiation (“CCRT”) and recruitment is ongoing NSCLC Amenable to Re-irradiation (Phase 1 Study MDA 2020-0123 sponsored by MD Anderson) Completed the dose escalation part of a Phase 1 study evaluating JNJ-1900 (NBTXR3) for patients with inoperable, recurrent NSCLC amenable to re-irradiation (“reRT”) and established the recommended Phase 2 dose (RP2D) at 33% of gross tumor volume in 4Q2024 Presented first data from the Phase 1 NSCLC reRT study showing a favorable safety profile and early signals of efficacy in 1Q2025 Lead programs in head and neck cancer and lung cancer proceeding as planned Ongoing Pivotal Phase 3 LA-HNSCC Study (NANORAY-312) Aligned on intent to transfer the global NANORAY-312 sponsorship from Nanobiotix to Janssen Pharmaceutica NV, a Johnson & Johnson Company (“Johnson & Johnson”) in 2Q2024 to begin preparations for interim analysis. Completed the NANORAY-312 US sponsorship transfer from Nanobiotix to Johnson & Johnson, in 4Q2024, with intent to transfer NANORAY-312 globally to Johnson & Johnson by 3Q2025. Ongoing Randomized Phase 2 Study in Unresectable Stage 3 NSCLC (CONVERGE) First patient dosed in the Johnson & Johnson-sponsored Phase 2 randomized CONVERGE study evaluating JNJ-1900 (NBTXR3) for patients with unresectable stage 3 non-small cell lung cancer (“NSCLC”) in 1Q2025 Multiple early-stage studies across solid tumor types beyond the lead programs continue to progress RM-HNSCC (Phase 1 Study 1100 sponsored by Nanobiotix) Presented new data showing disease control and tumor response in patients treated with JNJ-1900 (NBTXR3) followed by anti-PD-1 as a second-or-later line (2L+) therapy for patients with recurrent or metastatic (“RM”) head and neck squamous cell carcinoma (“HNSCC”) in 2Q2024 Locally Advanced or Borderline Resectable Pancreatic Cancer (Phase 1 Study MDA 2019-1001 sponsored by The University of Texas MD Anderson Cancer Center) Completed a Phase 1 study evaluating JNJ-1900 (NBTXR3) for patients with locally advanced or borderline resectable pancreatic cancer in 4Q2024 and investigators recommended that the encouraging oncologic outcomes coupled with a favorable safety profile warrant further exploration in a randomized study US FDA approved a protocol amendment to the completed Phase 1 pancreatic cancer study in 4Q2024 enabling the launch of a new cohort designed to evaluate JNJ-1900 (NBTXR3) combined with standard-of-care concurrent chemoradiation (“CCRT”) and recruitment is ongoing NSCLC Amenable to Re-irradiation (Phase 1 Study MDA 2020-0123 sponsored by MD Anderson) Completed the dose escalation part of a Phase 1 study evaluating JNJ-1900 (NBTXR3) for patients with inoperable, recurrent NSCLC amenable to re-irradiation (“reRT”) and established the recommended Phase 2 dose (RP2D) at 33% of gross tumor volume in 4Q2024 Presented first data from the Phase 1 NSCLC reRT study showing a favorable safety profile and early signals of efficacy in 1Q2025 Ongoing Pivotal Phase 3 LA-HNSCC Study (NANORAY-312) Aligned on intent to transfer the global NANORAY-312 sponsorship from Nanobiotix to Janssen Pharmaceutica NV, a Johnson & Johnson Company (“Johnson & Johnson”) in 2Q2024 to begin preparations for interim analysis. Completed the NANORAY-312 US sponsorship transfer from Nanobiotix to Johnson & Johnson, in 4Q2024, with intent to transfer NANORAY-312 globally to Johnson & Johnson by 3Q2025. Ongoing Randomized Phase 2 Study in Unresectable Stage 3 NSCLC (CONVERGE) First patient dosed in the Johnson & Johnson-sponsored Phase 2 randomized CONVERGE study evaluating JNJ-1900 (NBTXR3) for patients with unresectable stage 3 non-small cell lung cancer (“NSCLC”) in 1Q2025 Aligned on intent to transfer the global NANORAY-312 sponsorship from Nanobiotix to Janssen Pharmaceutica NV, a Johnson & Johnson Company (“Johnson & Johnson”) in 2Q2024 to begin preparations for interim analysis. Completed the NANORAY-312 US sponsorship transfer from Nanobiotix to Johnson & Johnson, in 4Q2024, with intent to transfer NANORAY-312 globally to Johnson & Johnson by 3Q2025. First patient dosed in the Johnson & Johnson-sponsored Phase 2 randomized CONVERGE study evaluating JNJ-1900 (NBTXR3) for patients with unresectable stage 3 non-small cell lung cancer (“NSCLC”) in 1Q2025 RM-HNSCC (Phase 1 Study 1100 sponsored by Nanobiotix) Presented new data showing disease control and tumor response in patients treated with JNJ-1900 (NBTXR3) followed by anti-PD-1 as a second-or-later line (2L+) therapy for patients with recurrent or metastatic (“RM”) head and neck squamous cell carcinoma (“HNSCC”) in 2Q2024 Locally Advanced or Borderline Resectable Pancreatic Cancer (Phase 1 Study MDA 2019-1001 sponsored by The University of Texas MD Anderson Cancer Center) Completed a Phase 1 study evaluating JNJ-1900 (NBTXR3) for patients with locally advanced or borderline resectable pancreatic cancer in 4Q2024 and investigators recommended that the encouraging oncologic outcomes coupled with a favorable safety profile warrant further exploration in a randomized study US FDA approved a protocol amendment to the completed Phase 1 pancreatic cancer study in 4Q2024 enabling the launch of a new cohort designed to evaluate JNJ-1900 (NBTXR3) combined with standard-of-care concurrent chemoradiation (“CCRT”) and recruitment is ongoing NSCLC Amenable to Re-irradiation (Phase 1 Study MDA 2020-0123 sponsored by MD Anderson) Completed the dose escalation part of a Phase 1 study evaluating JNJ-1900 (NBTXR3) for patients with inoperable, recurrent NSCLC amenable to re-irradiation (“reRT”) and established the recommended Phase 2 dose (RP2D) at 33% of gross tumor volume in 4Q2024 Presented first data from the Phase 1 NSCLC reRT study showing a favorable safety profile and early signals of efficacy in 1Q2025 Presented new data showing disease control and tumor response in patients treated with JNJ-1900 (NBTXR3) followed by anti-PD-1 as a second-or-later line (2L+) therapy for patients with recurrent or metastatic (“RM”) head and neck squamous cell carcinoma (“HNSCC”) in 2Q2024 Completed a Phase 1 study evaluating JNJ-1900 (NBTXR3) for patients with locally advanced or borderline resectable pancreatic cancer in 4Q2024 and investigators recommended that the encouraging oncologic outcomes coupled with a favorable safety profile warrant further exploration in a randomized study US FDA approved a protocol amendment to the completed Phase 1 pancreatic cancer study in 4Q2024 enabling the launch of a new cohort designed to evaluate JNJ-1900 (NBTXR3) combined with standard-of-care concurrent chemoradiation (“CCRT”) and recruitment is ongoing Completed the dose escalation part of a Phase 1 study evaluating JNJ-1900 (NBTXR3) for patients with inoperable, recurrent NSCLC amenable to re-irradiation (“reRT”) and established the recommended Phase 2 dose (RP2D) at 33% of gross tumor volume in 4Q2024 Presented first data from the Phase 1 NSCLC reRT study showing a favorable safety profile and early signals of efficacy in 1Q2025 Disciplined Financial Strategy Toward Long-Term Sustainability and Growth Received $20M first milestone payment related to NANORAY-312 from Johnson & Johnson in May 2024 Executed amendment to global licensing agreement for JNJ-1900 (NBTXR3) in 1Q2025, removing the vast majority Nanobiotix funding obligation for NANORAY-312 and releasing Johnson & Johnson from select future potential milestone payments, while safeguarding Nanobiotix’s path to sustainable cashflow through hundreds of millions in potential milestone payments related to lead programs expected in the coming years Extended cash runway to mid-2026 with a meaningful reduction in cash burn expected immediately and moving forward through and beyond mid-2026 Actively exploring preferably non-dilutive financing options to further extend cash visibility into 2027 Other Operational Highlights Strengthened Supervisory Board with the nominations of Dr. Margaret A. Liu and Ms. Anat Naschitz as board observers in 3Q2024, two key additions intended to further equip the Company for sustainable long-term growth. Dr. Liu brings a wealth of experience in US and international academia, pharmaceuticals, biotechnology and public policy Ms. Naschitz brings world-class expertise in raising and deploying capital to support disruptive innovation for the benefit of patients, healthcare professionals and investors Launched the Curadigm Nanoprimer Platform, the Company’s next lever for growth, in 4Q2024 The platform consists of nanoparticles designed with specific physico-chemical properties that allow temporary occupation of the liver cells responsible for therapeutic clearance. This mechanism is intended to increase the blood bioavailability and subsequent accumulation of therapeutics in the targeted tissues, potentially providing the opportunity to increase the efficacy or decrease the toxicity of intravenously administered medicines. As such, it is a potentially ideal technology to be used by both Nanobiotix and potential partners. Received $20M first milestone payment related to NANORAY-312 from Johnson & Johnson in May 2024 Executed amendment to global licensing agreement for JNJ-1900 (NBTXR3) in 1Q2025, removing the vast majority Nanobiotix funding obligation for NANORAY-312 and releasing Johnson & Johnson from select future potential milestone payments, while safeguarding Nanobiotix’s path to sustainable cashflow through hundreds of millions in potential milestone payments related to lead programs expected in the coming years Extended cash runway to mid-2026 with a meaningful reduction in cash burn expected immediately and moving forward through and beyond mid-2026 Actively exploring preferably non-dilutive financing options to further extend cash visibility into 2027 Strengthened Supervisory Board with the nominations of Dr. Margaret A. Liu and Ms. Anat Naschitz as board observers in 3Q2024, two key additions intended to further equip the Company for sustainable long-term growth. Dr. Liu brings a wealth of experience in US and international academia, pharmaceuticals, biotechnology and public policy Ms. Naschitz brings world-class expertise in raising and deploying capital to support disruptive innovation for the benefit of patients, healthcare professionals and investors Launched the Curadigm Nanoprimer Platform, the Company’s next lever for growth, in 4Q2024 The platform consists of nanoparticles designed with specific physico-chemical properties that allow temporary occupation of the liver cells responsible for therapeutic clearance. This mechanism is intended to increase the blood bioavailability and subsequent accumulation of therapeutics in the targeted tissues, potentially providing the opportunity to increase the efficacy or decrease the toxicity of intravenously administered medicines. As such, it is a potentially ideal technology to be used by both Nanobiotix and potential partners. Dr. Liu brings a wealth of experience in US and international academia, pharmaceuticals, biotechnology and public policy Ms. Naschitz brings world-class expertise in raising and deploying capital to support disruptive innovation for the benefit of patients, healthcare professionals and investors The platform consists of nanoparticles designed with specific physico-chemical properties that allow temporary occupation of the liver cells responsible for therapeutic clearance. This mechanism is intended to increase the blood bioavailability and subsequent accumulation of therapeutics in the targeted tissues, potentially providing the opportunity to increase the efficacy or decrease the toxicity of intravenously administered medicines. As such, it is a potentially ideal technology to be used by both Nanobiotix and potential partners. ____________________1 Potential first-in-class radioenhancer “NBTXR3” was licensed to Johnson & Johnson by Nanobiotix in 2023 and renamed “JNJ-1900” for the purposes of Johnson & Johnson-led clinical development. “2024 was a productive year focused on strong clinical execution and establishing a foundation for the next stage of growth. We expanded into lung cancer with the dosing of the first patient in the CONVERGE Phase 2 trial evaluating the potential of JNJ-1900 (NBTXR3) to enhance the treatment of first-line Stage III lung cancer patients.” said Laurent Levy, co-founder of Nanobiotix and chairman of the executive board. “Conviction in the broad potential of JNJ-1900 (NBTXR3) was further bolstered by the prolonged survival and well-tolerated safety reported across three key programs including Phase 1 data from Study 1100 evaluating NBTXR3 in combination with an anti-PD-1 in recurrent or metastatic head and neck cancer, as well as initial data supporting expansion potential in pancreatic and lung cancer as part of our MD Anderson collaboration. We also introduced our next generation first-in-class nanotherapeutic platform, Curadigm, late in 2024, which we expect to drive additional long-term value. With our financial position strengthened and new data this year in head and neck, lung, pancreatic, and esophageal cancer, we believe Nanobiotix is strongly positioned for impact in 2025 and beyond.” Full Year 2024 Financial Results Revenue and Other Income: Negative Revenues of €7.2 million were recognized in 2024, compared to €36.2 million for the year ended December 31, 2023, in which year significant revenue was recorded in connection with the execution of the license agreement with Janssen as well as the recognition of a development milestone. The negative non-cash revenue impact recognized in 2024 results from the transfer of NANORAY-312 study sponsorship to Janssen, signed at the end of 2024, which amounts to negative €19.3 million and is driven by a one-time recognition of a net liability towards Janssen to reflect this new situation. This one-off, non-cash negative revenue item, that results from application of IFRS 15 revenue recognition accounting principles, is partially offset by Other Revenues that do positively impact Nanobiotix’s cash position including: Sales of Clinical Products and goods to Janssen for €5.9 million, Technology Transfer Services billed to Janssen for €1.8 million, Research Tax Credit for €3.3 million and other income related to collaboration and supply services agreement in Asian territory for €1.0 million. Research and Development (“R&D”) Expenses: R&D expenses consist primarily of preclinical, clinical, and manufacturing expenses related to the development of NBTXR3 (JNJ-1900) and totaled €40.5 million for the twelve-month period ended December 31, 2024, as compared to €38.4 million for the twelve months ended December 31, 2023, a year over year increase of 5%. The increase in net R&D expenses was primarily due to an increase of clinical development activities, driven by the costs related to NANORAY-312 and of its Phase 1 multi-cohort trial of RT-activated NBTXR3 followed by anti-PD-1 checkpoint inhibitors (Study 1100), and the full-year impact of R&D positions that were recruited in 2023. Selling, General and Administrative (“SG&A”) Expenses: SG&A expenses were €20.5 million for the year ended December 31, 2024, compared to €22.0 million for the year ended December 31, 2023. This year-over-year decrease of 7% is mainly due to one-off fees incurred in 2023, consisting of license agreement execution and equity issuance related legal expenses and next to one-off fees paid to a financial advisor for €1.9 million in total. Net loss: Net loss attributable to shareholders was €68.1 million, a year-over-year increase of 72%, or €1.44 per share, for the twelve-month period ended December 31, 2024, which was primarily attributable to the one-off non-cash negative revenue recognition accounting impact. This compares to a net loss of €39.7 million, or €1.08 per share for the year ended December 31, 2023. Cash and Cash Equivalents: As of December 31, 2024, Nanobiotix had €49.7 million in cash and cash equivalents, compared to €75.3 million as of December 31, 2023. Financial Guidance: Based on the current operating plan and financial projections, Nanobiotix anticipates that the cash and cash equivalents of €49.7 million as of December 31, 2024 will fund its operations into mid-2026. The supervisory board of the Company has reviewed the financial statements 2024 on April 2nd, 2025 and the Company’s statutory auditors have finalized their audit and issued on March 31, 2025 a clean opinion on both statutory and consolidated financial statements 2024. “We have recently fortified our financial position and extended our cash runway into mid-2026 by amending our global agreement with Johnson & Johnson. We expect this action to reduce operational burn with immediate effect and this reduction will continue post mid-2026,” said Bart Van Rhijn, chief financial and business officer at Nanobiotix. “This non-dilutive financing solution achieved via the amendment, which we believe signals the increasing shared commitment to JNJ-1900 (NBTXR3), positions us to continue taking the necessary operational and financial steps to ensure the future of our business and our potential to benefit millions of patients around the world with our technology and signifies our continued financial discipline.” Availability of the Full Year 2024 Financial Reports The URD and 20-F are available on the Nanobiotix website here. In addition, the URD is available on the AMF website (www.amf-france.org) and the 20-F is available on the SEC website (www.sec.gov). The Company’s 2024 URD includes its: 2024 annual financial report including the report on corporate governance Reports from the Company’s statutory auditors and information on their feesRequired information in relation to the Company’s share buyback program Conference Call and Webcast Nanobiotix will host a conference call and live audio webcast on Thursday, April 3, 2025, at 8:00 AM EDT / 2:00 PM CEST, prior to the open of the U.S. market. During the call, Laurent Levy, chief executive officer, and Bart van Rhijn, chief financial and business officer, will briefly review the Company’s year-end results and provide an update on business activities for the full year of 2024 before taking questions from participants. Details of the call are as follows: Webcast link: click here Conference call link: click here Details of the call are also available in the investors section of the Company’s website at www.nanobiotix.com. It is recommended to join 10 minutes prior the event start. A replay of the webcast will be available shortly after the conclusion of the call and will be archived on the Company’s website. Participants are invited to email their questions in advance to investors@nanobiotix.com. About NBTXR3 (JNJ-1900) NBTXR3 (JNJ-1900) is a novel, potentially first-in-class oncology product composed of functionalized hafnium oxide nanoparticles that is administered via one-time intratumoral injection and activated by radiotherapy. Its proof-of-concept was achieved in soft tissue sarcomas for which the product received a European CE mark in 2019. The product candidate’s physics-based mechanism of action (MoA) is designed to induce significant tumor cell death in the injected tumor when activated by radiotherapy, subsequently triggering adaptive immune response and long-term anti-cancer memory. Given the physics-based MoA, Nanobiotix believes that NBTXR3 (JNJ-1900) could be scalable across any solid tumor that can be treated with radiotherapy and across any therapeutic combination, particularly immune checkpoint inhibitors. Radiotherapy-activated NBTXR3 (JNJ-1900) is being evaluated across multiple solid tumor indications as a single agent or in combination with anti-PD-1 immune checkpoint inhibitors, including in NANORAY-312—a global, randomized Phase 3 study in locally advanced head and neck squamous cell cancers. In February 2020, the United States Food and Drug Administration granted regulatory Fast Track designation for the investigation of NBTXR3 (JNJ-1900) activated by radiation therapy, with or without cetuximab, for the treatment of patients with locally advanced HNSCC who are not eligible for platinum-based chemotherapy—the same population being evaluated in the Phase 3 study. Given the Company’s focus areas and balanced against the scalable potential of NBTXR3 (JNJ-1900), Nanobiotix has engaged in a collaboration strategy to expand development of the product candidate in parallel with its priority development pathways. Pursuant to this strategy, in 2019 Nanobiotix entered into a broad, comprehensive clinical research collaboration with The University of Texas MD Anderson Cancer Center to sponsor several Phase 1 and Phase 2 studies evaluating NBTXR3 (JNJ-1900) across tumor types and therapeutic combinations. In 2023 Nanobiotix announced a license agreement for the global co-development and commercialization of NBTXR3 (JNJ-1900) with Janssen Pharmaceutica NV, a Johnson & Johnson company. About NANOBIOTIX Nanobiotix is a late-stage clinical biotechnology company pioneering disruptive, physics-based therapeutic approaches to revolutionize treatment outcomes for millions of patients; supported by people committed to making a difference for humanity. The Company’s philosophy is rooted in the concept of pushing past the boundaries of what is known to expand possibilities for human life. Incorporated in 2003, Nanobiotix is headquartered in Paris, France and is listed on Euronext Paris since 2012 and on the Nasdaq Global Select Market in New York City since December 2020. The Company has subsidiaries in Cambridge, Massachusetts (United States) amongst other locations. Nanobiotix is the owner of more than 25 patent families associated with three (3) nanotechnology platforms with applications in 1) oncology; 2) bioavailability and biodistribution; and 3) disorders of the central nervous system. For more information about Nanobiotix, visit us at www.nanobiotix.com or follow us on LinkedIn and Twitter. Disclaimer This press release contains “forward-looking” statements within the meaning of the “safe harbor” provisions of the Private Securities Litigation Reform Act of 1995, including, but not limited to, statements regarding the use of proceed therefrom, and the period of time through which the Company’s anticipates its financial resources will be adequate to support operations. Words such as “expects”, “intends”, “can”, “could”, “may”, “might”, “plan”, “potential”, “should” and “will” or the negative of these and similar expressions are intended to identify forward-looking statements. These forward-looking statements which are based on the Company’ management’s current expectations and assumptions and on information currently available to management. These forward-looking statements involve known and unknown risks, uncertainties and other factors that could cause actual results to differ materially from those implied by the forward-looking statements, including risks related to Nanobiotix’s business and financial performance, which include the risk that assumptions underlying the Company’s cash runway projections are not realized. Further information on the risk factors that may affect company business and financial performance is included in Nanobiotix’s Annual Report on Form 20-F filed with the SEC on April 2, 2025 under “Item 3.D. Risk Factors”, in Nanobiotix’s 2024 universal registration document filed with the AMF on April 2, 2025, and subsequent filings Nanobiotix makes with the SEC from time to time which are available on the SEC’s website at www.sec.gov. The forward-looking statements included in this press release speak only as of the date of this press release, and except as required by law, Nanobiotix assumes no obligation to update these forward-looking statements publicly. Contacts Attachment 2025-04-02 -- NBTX -- FY24 Financial Results -- FINAL.html