"Shilajit" is a panacea in Ayurveda, the Indian traditional system of medicine. The major bioactives of "shilajit" have been identified as dibenzo-a-pyrones (DBPs), its oligomers and aminoacyl conjugated derivatives These bioactive compounds play a crucial role in energy metabolism in all animal cells including those of man. 3-Hydroxydibenzo-α-pyrone (3-OH-DBP), a key DBP component of "shilajit" is converted, among other products, to another active DBP derivative, viz. 3,8-hydroxydibenzo-α-pyrone, 3,8(OH)2-DBP, in vivo, when its precursor is ingested. 3,8(OH)2-DBP is then involved in energy synthesis in the mitochondria in the reduction and stabilization of coenzyme Q10 in the electron transport chain. As the chem. synthesis of 3,8(OH)2-DBP is a complex, multi-step process and economically not readily viable, we envisioned the development of a process using microorganisms for bioconversion of 3-OH-DBP to 3,8(OH)2-DBP. In this study, the biotransformation of 3-OH-DBP is achieved using Aspergillus niger, which was involved in the humification process on sedimentary rocks leading to derivatives was achieved. The products were characterized and estimated by high performance liquid chromatog. (HPLC), high performance flash chromatog. (HPFC) and gas chromatog.-mass spectrometry (GC-MS) analyses. Among the Aspergillus species isolated and identified from native "shilajit", A. niger was found to be the most efficient for this bioconversion.