Shanxi Kangbao Biological Product Co. Ltd.

Objective To establish a new method for the determining the contents of tri-Bu phosphate in the human coagulation factor VIII.Methods The content of tri-Bu phosphate was determined by gas chromatog.Results Under the selected experiment conditions, there was a good linear relationship for the content of tri-Bu phosphate in the range of 2-16μg/L.The RSD was 0.11% in the accuracy test.The RSD was 9.6% in the repeatability test.The average recovery rate was 91% with RSD 5.1%.Conclusion The method is accurate to determine the contents of tri-Bu phosphate in the human Coagulation Factor VIII, and can be used to determine the contents of tri-Bu phosphate in the human Coagulation Factor VIII.

Objective To establish a method for the determination of antimony content in human prothrombin complex.Methods The content of antimony in human prothrombin complex was determined by hydride generation-at. absorption spectrometry.Results The calibration curve of antimony was linear in the range of 0-25.0 ng/mL with correlation coefficient higher than 0.99.The detection limit was 0.553 3 ng/mL and the quant. limit was 1.844 ng/mL.The recoveries were 95.93%-118.62%.Conclusion The method is simple,rapid,accurate and sensitive,and can be suitable for the determination of antimony in human prothrombin complex.

The effect of sheep red blood cells from different geog. origins and the titer of complement control on the anticomplementary activity in human i.v. Igs (PH4) (IVIG) was investigated.The CH50 method was used to determine the anticomplementary activity in human i.v. Igs.Anticomplementary activity in IVIG was determined with sheep red blood cells different geog. origins.Anticomplementary activity in IVIG was determined with different titers of complement controls.The sheep red blood cells from different titers of complement controls influenced the anticomplementary activity in IVIG.The sheep red blood cells from different geog. origins and complements with different titers may interfere the measurement of anticomplementary activity, thereby causing some deviations of the results.