Objective To establish a method for sterility test of the triazole antifungal drug posaconazole injection. Methods Based on the verification test of sterility test,the establishment of sterility test method was completed through the whole process of washing solution,filter material,washing quantity,selection of pos. control bacteria and adding sequence. Results Membrane filtration method was used for sterility test of posaconazole injection. During the test,Candida albicans was used as pos. control bacteria,SBECD solution( pH 3. 5) was used as washing solution,whose total amount was 1 000 m L. Conclusion This method is feasible and reliable for the detection of triazole antifungal drugs such as posaconazole injection.