Objective: To determine geniposide in Gardenia jasminoides extract by HPLC. Methods: Chromatog. column was Dima C18 (250mm × 4.6mm × 5μm); column temperature was 40°C; the mobile phase was composed of methanol, water and phosphoric acid; the detection wavelength was 238nm. Results: The linearity was good in a range of 24.6-98.4 mg/mL, with an average recovery of 99.1%. Conclusion: HPLC is reliable for determination of geniposide in Gardenia jasminoides extract