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This paper established the determination of salvianic acid A and protocatechuic aldehyde in compound Danshen dripping pills by HPLC at one time.The detection condition was as follows: C18 column, methanol-water-glacial acetic acid (19:80:1) as mobile phase, and detect wavelength of 279 nm.The results showed that salvianic acid A and protocatechuic aldehyde had good linear relation in range of 10-80 mg/L and 2-16 mg/L, the average recovery rates of salvianic acid A and protocatechuic aldehyde were 99.5% and 99.7%, and RSD were 1.6% and 1.3% resp.In conclusion, this method was simple, sensitive and accurate with easy sample disposal, and can be used as quality control method for compound Danshen dripping pills.

Analytical Methods

Girard reagent purification coupled with hollow-fiber-based stirring liquid microextraction followed by HPLC with diode array detection for determination of naphthoquinones in Qing Long Yi

作者: Jiang, Yanqiu ; Wang, Xiaoli ; Liu, Chenggang ; Zhou, Yuanyuan ; Liu, Yuxin ; Niu, Feng ; Yang, Bingyou ; Kuang, Haixue

Naphthoquinones all with carbonyl groups may react with the Girard reagent to form a water-soluble substance called hydrazone. These denatured naphthoquinones can recover lipid solubility transferred to the organic liquid layer.

Heilongjiang Yiyao

Separation of toxic alkaloids from Aconitum carmichaeli

作者: Tang, Qingxu

A method was established for extracting and separating mesaconitine, aconitine and hypaconitine.The recovery of this method was similar to that of alk. benzene method.This method was at low cost, harmless and suitable for extracting, separating and preparing mesaconitine, aconitine and hypaconitine.

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