Staphylococcus aureus (S. aureus) infection had become the world's problem, its resistance and spread rapidly growing its harm. In vaccine research of S. aureus, some surface protein of S. aureus had good immunogenicity, and could be used as a vaccine candidate. IsdB was a kind of surface proteins, and had good immunogenicity, and its antigen epitope-based determined on the basis of this protein for a single protein vaccines or fusion protein vaccine had great significance for research and preparation This paper took IsdB monoclonal antibodies to 3B6 McAb strains for ligands, from random peptide library phage antibody screening in seven simulation of recognition. After the sequencing and sequence alignment anal., it was found that epitope of IsdB antigens was based for space conformation, it concentrated in curriculum-based front-end. This research had certain reference value for IsdB vaccine research.