The paper determined chlorogenic acid in pediatric Kechuanling granule by HPLC. The HPLC was performed on a Shim-pack-C18 column with acetonitrile-0.4% phosphoric acid (11 : 89) as mobile phase, at a wavelength of 327 nm. The results showed that the linearity was in the range of 0.2176-1.088 μg (r = 0.9994), and the recovery rate was 98.23% (RSD = 0.97%). The method was simple, accurate, and sensitive, and could be used for the quality control of the pediatric Kechuanling granule.