Not-celiac Wheat Sensitivity (NCWS) in Patients With Irritable Bowel Syndrome. Randomized Double-Blind Placebo-Controlled Trial (Wheat vs Placebo) for Bio-markers Identification of NCWS and Understanding Its Pathogenetic Mechanisms.

The aim of the investigators' study is to evaluate biochemical, immunological and histological characteristics of patients affected with the so-called "gluten (or wheat) sensitivity" who suffers from irritable bowel syndrome (IBS)-like symptoms.
As it is not known what component of the cereals causes the symptoms in so called "gluten-sensitive" patients, the investigators prefer to speak of "not-celiac wheat sensitivity" (NCWS).
NCWS patients may be defined as ones, neither celiac or allergic to wheat, who develop symptoms following wheat consumption, that improved on wheat/gluten free diet (GFD). For our research, we will select adult patients, both genders, affected with suspected NCWS (i.e. with symptoms/signs which disappeared on GFD and worsen on a gluten containing diet, testing negative for celiac disease [anti-tissue transglutaminase antibodies, anti-tTG, and anti-endomysium antibodies, EMA, and with biopsy Marsh 0-1] and wheat allergy [serum specific IgE for wheat]). The patients will be recruited at the Department of Internal Medicine, 'Giovanni Paolo II' Hospital of Sciacca (Agrigento), and of Internal Medicine of the University of Palermo, from January 2012 to October 2013, for IBS-like symptoms.
At the time of the recruitment, the patients will be on GFD by at least one month and must be asymptomatic. A more restricted elimination diet (with the exclusion of cow's milk, egg and other foods) could be prescribed in patients who are suspected to suffer from multiple food hypersensitivity. The patients will be randomized to undergo a double-blind placebo-controlled study, assuming wheat flour or placebo, administered daily for 15 days. Before and after the challenge, the investigators will evaluate gastrointestinal (Gastrointestinal Symptom Rating Scale, GSRS) and the investigators will collect blood and fecal sampling and biopsies from endoscopic evaluation (both esophagogastroduodenoscopy and rectoscopy, with multiple biopsies), for the identification of possible markers (serological, biochemical, immunological, histological features, expression of cytokines and other constitutive mucosal proteins from peripheral blood mononuclear cells, mucosal lymphocytes and fecal biomarkers) that may be of help to diagnose the condition of NCWS and to understand its pathogenesis.

开始日期2012-01-01

申办/合作机构

University of Palermo

NCT01762566

/ CompletedN/AIIT

Randomized Double-Blind Placebo-Controlled Trial (Wheat vs Placebo) in Wheat Sensitive Patients for Evaluation of Bio-Markers of a Single Referred Symptom (Bloating & Swelling)

Aim of the investigators' study is to evaluate one of the symptoms described by patients affected with the so-called "not-celiac wheat sensitivity" (NCWS). NCWS patients may be defined as ones, neither celiac or allergic to wheat, who develop symptoms following wheat consumption and improve on Gluten Free Diet (GFD). Among these, patients often report the appearance of bloating and abdominal pain, and, sometimes, swelling of fingers and face, shortly after ingestion of wheat. They also refer that swelling tends to last for some hours and then to disappear on GFD. To our knowledge, this disorder has never been investigated neither proved.
For the investigators' research, the investigators selected adult patients, both genders, affected with NCWS. Diagnostic criteria include: 1) symptoms disappearance on GFD, 2) testing negative for celiac disease [anti-tTG and EMA, and with biopsy Marsh 0-1] and wheat allergy [serum specific IgE for wheat], 3) positive response to a double-blind placebo-controlled challenge (DBPC) with wheat or placebo.
Patients will be recruited among subjects referring, as outpatients, to the Department of Internal Medicine, 'Giovanni Paolo II' Hospital of Sciacca (Agrigento), and of Internal Medicine of the University of Palermo, from January 2012 to October 2013. The investigators will include patients referring for functional dyspepsia and/or irritable bowel syndrome (IBS)-like symptoms. All recruited patients must report body's swelling immediately after wheat ingestion. In all recruited patients, NCWS diagnosis was posed during the previous years, according to the above mentioned criteria.
Patients, all on GFD for at least 30 days, will undergo to another DBPC with wheat or placebo (xylose), performed by administering capsules coded as A or B containing wheat or xylose, respectively. Capsules A or B will be administered once, and then, after 1 week of washout, patients received the other capsules, given once too. Before and 2 hours after every challenge, patients will undergo a complete medical examination (measurement of body weight, height, body mass index [BMI], waist circumference, diameter of thighs, legs, arms, and fingers), and we will perform bioelectrical impedance analysis and collect blood sampling, for identification of possible markers (bioelectrical, immunologic, hormonal, etc) that may help to demonstrate and explain mechanisms of examined symptom.

开始日期2012-01-01

申办/合作机构

University of Palermo

ACTRN12612001131842

/ CompletedN/AIIT

A randomised placebo controlled crossover trial to evaluate the effects of a D-xylose preload, with or without sitagliptin, on incretin hormone secretion, gastric emptying, and postprandial glycaemia in patients with type 2 diabetes mellitus.

开始日期2011-11-01

申办/合作机构

Royal Adelaide Hospital

100 项与 Xylose 相关的临床结果

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100 项与 Xylose 相关的转化医学

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47,906

项与 Xylose 相关的文献（医药）

2025-09-01·TALANTA

One-stop solution for wide polar range compounds: Preparation and application of quaternary ammonium salt molecular cage stationary phase

Article

作者: Li, Zhen ; Lan, Tao ; Pei, Dong ; Liu, Sheng ; Zhu, He ; Ding, Ruifang ; Lv, Mei ; Wang, Litao ; Zhang, Yangyang ; Zuo, Ying ; Hu, Jinxia

The separation and analysis of complex samples with wide polar range on a singular column is always a difficult problem in separation and analysis science. In this study, the RCC3-R molecular cage modified with the quaternary ammonium salt stationary phase (RCC3-GQ@silica) was successfully prepared and applicated in the separation of wide polar range compounds. In the Reverse Phase Liquid Chromatography (RPLC) mode, due to the hydrophobicity and π-π interactions provided by the cyclohexane and benzene rings in the molecular cage structure, this stationary phase demonstrated effective separation capabilities for alkylbenzenes, polycyclic aromatic hydrocarbons, phenols, and anilines. In the Hydrophilic Interaction Liquid Chromatography (HILIC) mode, the study explored the separation performance of this stationary phase for sugars and inorganic salts. Utilizing a mixed mode of HILIC/RPLC/Ion Exchange Chromatography (IEC), effective separation was achieved for sulfonamides, nucleosides, acids, and amino acids, indicating good separation effects for medium to strongly polar compounds as well as various hydrophilic compounds. Combining various separation modes, the RCC3-GQ@silica stationary phase successfully separated 91 compounds across 15 categories. These results not only demonstrate the potential of the stationary phase in expanding the range of polarities of analyzable compounds and achieving multipurpose use on a single column, but also confirm its effective separation of nucleosides in pure water systems, further emphasizing the significant application potential of RCC3-GQ@silica stationary phase in the field of green chemistry.

2025-09-01·New Biotechnology

Enhancing 2,3-butanediol and acetoin production from brewer's spent grain hemicellulosic hydrolysate through bacterial co-cultivation

Article

作者: Coca, Mónica ; Dragone, Giuliano ; López-Linares, Juan C ; Perez, Caroline L ; García-Cubero, María Teresa ; Mussatto, Solange I ; Rama, Erlinda ; Yamakawa, Celina K

This study evaluated bacterial co-cultivation as a strategy to mitigate brewer's spent grain (BSG) hemicellulosic hydrolysate toxicity, aiming to enhance 2,3-butanediol (2,3-BDO) and acetoin production through fermentation. Co-culture of Paenibacillus polymyxa with Pseudomonas alloputida or Rhodococcus sp. was assessed using synthetic medium and BSG hydrolysate. Attention was given to removing inhibitory compounds, including lignin-derived phenolics, hydroxymethylfurfural, furfural, and acetic acid, through microbial detoxification during co-cultivation. Various fermentation temperatures (30, 34, and 37 °C) and initial cell concentrations (OD₆₀₀ of 0.05 and 0.1) were tested. Both P. polymyxa and Rhodococcus sp. effectively removed inhibitory compounds present in the medium. Co-cultures with Rhodococcus sp. exhibited higher sugar consumption rates (1.01 vs 0.88 g/L·h) than P. polymyxa monoculture, efficiently utilizing glucose, xylose, and arabinose, producing 2,3-BDO and acetoin. In co-culture with Rhodococcus sp., concentration (3.7 g/L), yield (0.14 g/g) and productivity (0.10 g/L·h) of 2,3-BDO at 34 °C considerably surpassed that of the P. polymyxa monoculture, with an increase of up to 48 %. These findings highlight the potential of co-cultures, especially with Rhodococcus sp., to alleviate inhibitory compound impacts when using complex media for fermentation. This study represents the first exploration of 2,3-BDO and acetoin production from BSG hemicellulosic hydrolysates using co-cultures.

2025-09-01·New Biotechnology

Valorization of waste cooking oil for bioproduction of industrially-relevant metabolites in Ashbya gossypii

Article

作者: Jiménez, Alberto ; Montero-Bullón, Javier-Fernando ; Buey, Rubén M ; Martín-González, Javier ; Muñoz-Fernández, Gloria

Waste cooking oil (WCO) is a byproduct of culinary processes, which undergoes degradation due to high temperatures during frying and cooking. Beyond its detrimental effects on health, including potential carcinogenic effects, WCO poses a significant environmental threat, emphasizing the need for urgent recycling efforts. Valorization of WCO as a carbon source for microbial fermentations emerges as a feasible alternative in a bioeconomy context. The aim of the present work is to explore the ability of Ashbya gossypii, a natural overproducer of riboflavin that is currently used in the industrial production of the vitamin, to exploit WCO for the production of industrially relevant metabolites such as riboflavin, folates, biolipids and monoterpenes. Our results demonstrate that WCO is an effective carbon source for A. gossypii bioproduction of riboflavin, folates and biolipids, reaching among the highest titers described so far in flask fermentation: riboflavin titer (312.5mg/L) increased 4.8-fold compared to glucose-based medium; folate production reached 7.6mg/L; and the intracellular lipids were above 80% of the cell dry weight. In contrast, the production of the monoterpenes limonene and sabinene was not improved with the utilization of WCO. Taken together, our results present a proof-of-principle for the implementation of a novel bioprocess for the valorization of WCO using the industrial fungus A. gossypii.

100 项与 Xylose 相关的药物交易

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研究	分期	人群特征	评价人数	分组	结果	评价	发布日期


EASD2016	N/A	-	-	FreeStyle Insulinx	襯膚蓋夢鹽築艱鹽範鹽(獵餘廠獵鹽選廠繭艱觸) = 築鑰鹹醖淵齋憲餘憲衊鏇壓餘簾鏇醖積鬱製選 (製膚衊鏇製鑰範築夢鬱 )	-	2016-09-14
EASD2012	N/A	2型糖尿病	10	Sitagliptin + Xylose	鏇窪憲鹹壓積鹽築簾壓(鹽願艱糧鬱遞憲製積構) = 夢壓選膚壓鏇鬱糧鬱糧齋構齋蓋繭鹽醖繭獵膚 (遞鹽廠鏇鏇願醖艱鹽窪 )	积极	2012-10-05
EASD2012	N/A	2型糖尿病	10	Sitagliptin + Control	鏇窪憲鹹壓積鹽築簾壓(鹽願艱糧鬱遞憲製積構) = 繭衊齋觸築衊網艱襯鏇齋構齋蓋繭鹽醖繭獵膚 (遞鹽廠鏇鏇願醖艱鹽窪 )	积极	2012-10-05