更新于：2024-11-01

CHIKV E1

更新于：2024-11-01

基本信息

别名-

简介-

关联

项与 CHIKV E1 相关的药物

PSSct1905

靶点

CHIKV E1

作用机制

CHIKV E1拮抗剂

在研机构

Universidade Estadual Paulista Júlio De Mesquita Filho Unesp

原研机构

Universidade Estadual Paulista Júlio De Mesquita Filho Unesp

在研适应症

基孔肯雅热

非在研适应症-

最高研发阶段临床前

首次获批国家/地区-

首次获批日期1800-01-20

CHIKV E2-E1-LNP mRNA vaccine

靶点

CHIKV E1 x CHIKV E2

作用机制

CHIKV E1拮抗剂 [+1]

在研机构

中国科学院上海巴斯德研究所

原研机构

中国科学院上海巴斯德研究所

在研适应症

基孔肯雅热

非在研适应症-

最高研发阶段临床前

首次获批国家/地区-

首次获批日期1800-01-20

MV-2CHIKV-3WIZ

靶点

CHIKV E1 x WNV E protein x ZIKV E protein

作用机制

CHIKV E1拮抗剂 [+2]

在研机构

Oncovita SAS

原研机构

Oncovita SAS

在研适应症

麻疹病毒感染

非在研适应症-

最高研发阶段临床前

首次获批国家/地区-

首次获批日期1800-01-20

100 项与 CHIKV E1 相关的临床结果

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100 项与 CHIKV E1 相关的转化医学

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0 项与 CHIKV E1 相关的专利（医药）

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项与 CHIKV E1 相关的文献（医药）

2020-12-01·Virology Journal3区 · 医学

Promising application of monoclonal antibody against chikungunya virus E1-antigen across genotypes in immunochromatographic rapid diagnostic tests

3区 · 医学

ArticleOA

作者: Nakayama, Emi E ; Leaungwutiwong, Pornsawan ; Huits, Ralph ; Phadungsombat, Juthamas ; van den Berg, Riemsdijk ; Cnops, Lieselotte ; Hasan, Abu ; Rahim, Rummana ; Shioda, Tatsuo ; Barbé, Barbara ; van Esbroeck, Marjan ; Rahman, Mizanur ; Tuekprakhon, Aekkachai ; Suzuki, Keita ; Iwamoto, Hisahiko

BACKGROUNDThree different genotypes of chikungunya virus (CHIKV) have been classified: East/Central/South African (ECSA), West African (WA), and Asian. Previously, a rapid immunochromatographic (IC) test detecting CHIKV E1-antigen showed high sensitivity for certain ECSA-genotype viruses, but this test showed poor performance against the Asian-genotype virus that is spreading in the American continents. We found that the reactivity of one monoclonal antibody (MAb) used in the IC rapid diagnostic test (RDT) is affected by a single amino acid substitution in E1. Therefore, we developed new MAbs that exhibited specific recognition of all three genotypes of CHIKV.METHODSUsing a combination of the newly generated MAbs, we developed a novel version of the IC RDT with improved sensitivity to Asian-genotype CHIKV. To evaluate the sensitivity, specificity, and cross-reactivity of the new version of the IC RDT, we first used CHIKV isolates and E1-pseudotyped lentiviral vectors. We then used clinical specimens obtained in Aruba in 2015 and in Bangladesh in 2017 for further evaluation of RDT sensitivity and specificity. Another alphavirus, sindbis virus (SINV), was used to test RDT cross-reactivity.RESULTSThe new version of the RDT detected Asian-genotype CHIKV at titers as low as 10^4 plaque-forming units per mL, a concentration that was below the limit of detection of the old version. The new RDT had sensitivity to the ECSA genotype that was comparable with that of the old version, yielding 92% (92 out of 100) sensitivity (95% confidence interval 85.0-95.9) and 100% (100 out of 100) specificity against a panel of 100 CHIKV-positive and 100 CHIKV-negative patient sera obtained in the 2017 outbreak in Bangladesh.CONCLUSIONSOur newly developed CHIKV antigen-detecting RDT demonstrated high levels of sensitivity and lacked cross-reactivity against SINV. These results suggested that our new version of the CHIKV E1-antigen RDT is promising for use in areas in which the Asian and ECSA genotypes of CHIKV circulate. Further validation with large numbers of CHIKV-positive and -negative clinical samples is warranted. (323 words).

2020-10-01·Antiviral Research2区 · 医学

Producing physicochemical property consensus alphavirus protein antigens for broad spectrum vaccine design

2区 · 医学

Article

作者: Baker, Wendy S ; Schein, Catherine H ; Braun, Werner ; Negi, Surendra

There is a pressing need for new vaccines against alphaviruses, which can cause fatal encephalitis (Venezuelan equine encephalitis virus (VEEV) and others) and severe arthralgia (e.g. Chikungunya virus, CHIKV). These positive-strand RNA viruses are diverse and evolve rapidly, meaning that the sequence of any vaccine should cover multiple strains that may be quite different from any previous isolate. Here, consensus proteins were produced to represent the common physicochemical properties (PCPs) of the epitope rich, B domain of the E2 envelope protein. PCP-consensus proteins were based on multiple strains of VEEV (VEEV_con) and CHIKV (CHIKV_con) or the conserved PCPs of 24 different alphaviruses (AllAV_con). The AllAV_con was altered to include binding sites for neutralizing antibodies of both VEEV and CHIKV strains (Mosaik_con). All four designed proteins were produced solubly in E. coli and purified. They formed the β-strand core expected from experimental structures of this region of the wild type E2 proteins as indicated by circular dichroism (CD) spectra. Furthermore, the CHIKV_con protein bound to a structure dependent, CHIKV neutralizing monoclonal antibody. The AllAV_con and Mosaik_con proteins bound to polyclonal antibodies generated during natural infection with either VEEV or CHIKV, indicating they contained epitopes of both serotypes. The Mosaik_con antigen induced antibodies in rabbit sera that recognized both the VEEV_con and CHIKV_con spike proteins. These PCP-consensus antigens are promising starting points for novel, broad-spectrum alphavirus vaccines.

2017-12-01·Virologica Sinica2区 · 医学

Detection, isolation, and characterization of chikungunya viruses associated with the Pakistan outbreak of 2016–2017

2区 · 医学

Article

作者: Jehan, Shoukat ; Zhang, Bo ; Li, Jun-Hua ; Deng, Cheng-Lin ; Zhang, Ya-Nan ; Wei, Hongping ; Jamil, Nadia ; Li, Xiao ; Deng, Fei ; Gao, Ai-Li ; Liu, Si-Qing

The chikungunya virus (CHIKV) is a mosquito-transmitted alphavirus, which has infected millions of people in Africa, Asia, Americas, and Europe since it reemerged in India and Indian Ocean regions in 2005-2006. Starting in the middle of November 2016, CHIKV has been widely spread, and more than 4,000 cases of infections in humans were confirmed in Pakistan. Here, we report the first isolation and characterization of CHIKV from the Pakistan outbreak. Eight CHIKV strains were newly isolated from human serum samples using a cell culture procedure. A full-length genome sequence and eight complete envelope (E1) sequences of CHIKV from Pakistan were obtained in this study. Alignment of the CHIKV E1 sequences revealed that the eight new CHIKV isolates were highly homogeneous, with only two nonsynonymous substitutions found at generally conserved sites (E99 and Q235). Based on the comparison of 342 E1 sequences, the two nonsynonymous mutations were located in well-recognized domains associated with viral functions such as the cell fusion and vector specificity, suggesting their potential functional importance. Phylogenetic analysis indicated that the CHIKV strains from Pakistan originated from CHIKV circulating in the Indian region. This study helps elucidate the epidemics of CHIKV in Pakistan and also provides a foundation for studies of evolution and expansion of CHIKV in South Asia.