The objective is to establish the method for determining the content of Me hesperidin in Weierkang capsule by high performance liquid chromatog. method: using C18 (150 mm × 4.6 mm, 5 μm) column, glacial acetic acid-methanol-water (5:25:70) as mobile phase, flow rate is 1.0 mL/min-1, detection wavelength is 283 nm. The mass concentration of Me hesperidin shows good linear relationship (r = 0.9999) in the range of 29.1-291 mg/L-1, the average recovery rate is 99.3%, RSD is 1.8% (n = 6). This method is simple, accurate, repeatability is good, and can be used in the quality control of Me hesperidin in Weierkang capsule.