更新于：2024-11-01

Hubei Provincial Hospital of TCM

中国北京市

更新于：2024-11-01

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项与 Hubei Provincial Hospital of TCM 相关的临床试验

NCT05439135 / Not yet recruitingN/AIIT

An Open-label, Multicenter, Randomized Clinical Trial for Malnutrition After Nonphysiological Reconstruction of the Upper Gastrointestinal Tract: Washed Microbiota Transplantation Via Mid-gut Tube Versus Colonic Transendoscopic Enteral Tube

This is a prospective, randomized, multicenter study to evaluate WMT in improving nutritional status in malnourished patients who underwent nonphysiological reconstruction of the upper gastrointestinal tract. In this multicenter trial, sixty-two patients will be enrolled in forteen Chinese sites. Participants will be randomized at a ratio of 1:1 to receive three WMTs through either mid-gut tube or colonic TET. After WMT, each participant will receive free diet plus home enteral nutrition. Home enteral nutrition should last for 2 months at home, with blood, urine and stool samples taken and stored at baseline and 2 months after WMT.

开始日期2024-10-20

申办/合作机构

兰州大学

[+9]

NCT06359418 / Not yet recruitingN/AIIT

Acupuncture for Obese Adults With Prediabetes: a Multicenter Randomized Controlled Trial

The clinical trial aims to evaluate the efficacy and safety of acupuncture on weight loss and changes of glucose and lipid metabolism in obese adults with pre-diabetes.

开始日期2024-04-10

申办/合作机构

中国中医科学院广安门医院

[+2]

ChiCTR2400079480 / SuspendedN/AIIT

To study the molecular mechanism of Anmeidan in preventing insomnia based on the regulation of astrocytes

开始日期2024-02-01

申办/合作机构

Hubei Provincial Hospital of TCM

100 项与 Hubei Provincial Hospital of TCM 相关的临床结果

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0 项与 Hubei Provincial Hospital of TCM 相关的专利（医药）

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210

项与 Hubei Provincial Hospital of TCM 相关的文献（医药）

2024-07-05·Medicine

Nonsurgical therapy for lumbar spinal stenosis caused by ligamentum flavum hypertrophy: A review

Review

作者: Wang, Zitong ; Mao, Tian ; Jiang, Jiecheng ; Fang, Nan ; Chen, Qian ; Yang, Aofei ; Wang, Zhigang

Lumbar spinal stenosis (LSS) can cause a range of cauda equina symptoms, including lower back and leg pain, numbness, and intermittent claudication. This disease affects approximately 103 million people worldwide, particularly the elderly, and can seriously compromise their health and well-being. Ligamentum flavum hypertrophy (LFH) is one of the main contributing factors to this disease. Surgical treatment is currently recommended for LSS caused by LFH. For patients who do not meet the criteria for surgery, symptom relief can be achieved by using oral nonsteroidal anti-inflammatory drugs (NSAIDs) and epidural steroid injections. Exercise therapy and needle knife can also help to reduce the effects of mechanical stress. However, the effectiveness of these methods varies, and targeting the delay in LF hypertrophy is challenging. Therefore, further research and development of new drugs is necessary to address this issue. Several new drugs, including cyclopamine and N-acetyl-l-cysteine, are currently undergoing testing and may serve as new treatments for LSS caused by LFH.

2024-06-01·Biochemical Genetics

5-Hydroxymethylfurfural Ameliorates Allergic Inflammation in HMC-1 Cells by Inactivating NF-κB and MAPK Signaling Pathways

Article

作者: Zhang, Zhuo ; Yu, Xiaolin ; Wang, Yinglin ; Hu, Pan

Allergic inflammation is the foundation of multiple allergic disorders, such as allergic rhinitis and asthma. Mast cells are effector cells that initiate inflammatory response. 5-hydroxymethylfurfural (5-HMF), a furfural compound, is the heat-processed product of various fruit, foods, drinks, as well as some Chinese herbal medicines. 5-HMF was previously reported to inhibit mast cell activation. Our study aimed to explore the functions of 5-HMF in both phorbol 12-mystate 13-acetate (PMA) plus calcium ionophore (A23187)-induced allergic inflammation in human mast cell line HMC-1 and ovalbumin (OVA)-induced asthma mouse models. HMC-1 cells were pretreated with 5-HMF and then stimulated by PMA+A23187. The cytotoxicity of 5-HMF on HMC-1 cells was evaluated by MTT assay. Histamine content in cell supernatants was measured by the o-phthaldialdehyde spectrofluorometric procedure. Intracellular calcium was determined using the fluorescent dye Fura-2AM. The production and expression of pro-inflammatory cytokines were detected by ELISA and RT-qPCR. Caspase-1 colorimetric assay was employed to examine the enzymatic activity of caspase-1. Asthma mouse models were induced by OVA sensitization. The bronchoalveolar lavage fluid (BALF) and blood samples were collected for the detection of total and differential cell count as well as aspartate aminotransferase (AST), alanine aminotransferase (ALT), OVA-immunoglobulin E (OVA-IgE), OVA-immunoglobulin G1 (OVA-IgG1), and pro-inflammatory cytokine levels. The left lung of mouse was dissected for histopathological examination by hematoxylin and eosin (H&E) staining. The protein expression of pro-caspase-1 and the phosphorylation of NF-κB and MAPK pathway-associated molecules were assessed by Western blotting. Our findings revealed that 5-HMF efficiently suppressed the PMA+A23187-induced enhancement in histamine production and intracellular calcium in HMC-1 cells. Pro-inflammatory cytokine production and expression in HMC-1 cells were elevated after PMA plus A23187 stimulation, which, however, were inhibited by pretreatment of 5-HMF. Additionally, 5-HMF suppressed the activity of caspase-1 and the phosphorylation of NF-κB and MAPK-associated molecules including p65 NF-κB, p38 MAPK, ERK, and JNK in HMC-1 cells. In vivo experiments demonstrated that 5-HMF treatment reduced the lung/body weight index and total and differential (macrophages, neutrophils, lymphocytes, and eosinophils) cell counts in BALF of asthmatic mice, but exerted no influence on serum AST and ALT levels. Besides, 5-HMF reduced serum OVA-IgE and OVA-IgG1 levels, mitigated lung inflammation, and inhibited the NF-κB and MAPK signaling pathways in asthma mouse models. 5-HMF mitigates allergic inflammation in asthma by inactivating caspase-1 and NF-κB and MAPK signaling pathways.

2024-04-12·Zhongguo zhen jiu = Chinese acupuncture & moxibustion

艾灸对肥胖大鼠肠道屏障功能及TLR4/NF-κB p65信号通路的影响.

Article

作者: Zhou, Zhongyu ; Kong, Weiqing ; Chen, Xia ; Huang, Wei ; Zhang, Yingrong ; Zhang, Yanji

目的: 观察艾灸对肥胖大鼠肠道屏障功能及Toll样受体4（TLR4）/核转录因子κB p65（NF-κB p65）信号通路的调控作用，探究艾灸干预肥胖的作用机制。方法: 将55只SPF级7周龄雄性Wistar大鼠随机分为正常组（10只）和造模组（45只），造模组采用高脂饮食喂养建立肥胖大鼠模型。造模成功的30只大鼠随机分为模型组、艾灸组、安慰对照组，每组10只。艾灸组在距离“中脘”3~5 cm处施灸，温度保持在（46±1）℃；安慰对照组在距离“中脘”8~10 cm处施灸，温度保持在（38±1）℃。均每天干预1次，共干预8周。观察各组大鼠干预前后体质量、摄食量；ELISA法测定各组大鼠血清总胆固醇（TC）、三酰甘油（TG）、脂多糖（LPS）水平，计算胰岛素抵抗指数（HOMA-IR）；HE染色法观察各组大鼠结肠组织形态；实时荧光定量PCR法检测各组大鼠结肠闭锁连接蛋白-1（ZO-1）、咬合蛋白（Occludin）、紧密连接蛋白-1（Claudin-1）、TLR4、NF-κB p65 mRNA表达；Western blot法检测各组大鼠结肠ZO-1、Occludin、Claudin-1、TLR4和NF-κB p65蛋白表达。结果: 与正常组比较，模型组大鼠体质量、摄食量、HOMA-IR及血清TC、TG、LPS水平升高（P<0.01）；与模型组和安慰对照组比较，艾灸组大鼠体质量、摄食量、HOMA-IR及血清TC、TG、LPS水平降低（P<0.01，P<0.05）。与正常组比较，模型组大鼠结肠组织黏膜大量上皮细胞损伤脱落，间质内可见明显炎性细胞浸润。与模型组比较，艾灸组大鼠结肠组织损伤有不同程度恢复，间质内可见少量炎性细胞浸润；安慰对照组大鼠结肠组织上皮损伤有少许恢复，间质内可见炎性细胞浸润。与正常组比较，模型组大鼠结肠ZO-1、Occludin、Claudin-1 mRNA和蛋白表达降低（P<0.01），结肠TLR4、NF-κB p65 mRNA和蛋白表达升高（P<0.01）；与模型组和安慰对照组比较，艾灸组大鼠结肠ZO-1、Occludin、Claudin-1 mRNA和蛋白表达升高（P<0.01，P<0.05），结肠TLR4、NF-κB p65 mRNA和蛋白表达降低（P<0.01）。结论: 艾灸可以降低肥胖大鼠体质量、摄食量，调节肥胖大鼠血脂，改善胰岛素抵抗，可能与改善肠道屏障功能、调节肠道TLR4/NF-κB p65信号通路来减轻炎性反应有关。.

100 项与 Hubei Provincial Hospital of TCM 相关的药物交易

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100 项与 Hubei Provincial Hospital of TCM 相关的转化医学

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