In order to optimize the formulation of Jiangu ointments, effects of several penetration enhancers on the permeation of dracorhodin through excised abdomen skin of rats were investigated.Franz-diffusion cell was adopted and the concentration of dracorhodin was determined by HPLC method.The enhancing effect on the permeation of dracorhodin of azone was more than those of propylene glycol, turpentine oil, oleic acid and menthol.Azone with the concentration of 2% had higher enhancing effect than those with the concentrations of 1%, 0.5% and 3%.