Objective: A HPLC method was established for the determination of paeoniflorin in Tiaojingquban Tablets.Methods: In the anal., a Diamonsil-C18 (150 mm × 4.6 mm, 5 μm) column, a mobile phase (25% methanol solution) and a UV wavelength of 230 nm.Results: A linear range of 0.0284 mg/mL-0.1988 mg/mL, an average recovery of 99.57% with RSD of 0.77% (n = 6).Conclusion: The method of HPLC is simple, rapid and reliable.