Character, identification, visible foreign matter, the solution color, pH, sterile, heat, loading capacity, 6,8-epitrioctic acid, related substances and their determination of lipoic acid injection were researched. HPLC was applied to determine lipoic acid in lipoic acid injection. Lipoic acid had good linear relationship within range of 50-1500 μg/mL, and there was high recovery rate. Content of lipoic acid injection was within 95.0%-105.0% of labeled amount Lipoic acid injection had good stability in 0.9% NaCl injection, and its content had less change within 6 h.