Jiangsu Chiatai Qingjiang Pharmaceutical Co., Ltd.

To set up HPLC methods for determining the water-soluble vitamins B₁, B₂, B₆ contents in compound vitamini B and belladonnae oral solutionZORBAX NH₂ column was used, acetonitrile: KH₂PO₄ (75:25) were used as mobile phase, while the flowing speed was 1.0 mL/min , detective wavelength at 280 nm, and column temperature was 40°C.The results indicated that the linear curve of tested vitamin B₁ concentration within the range of 1.21-18.48 μg/mL was ideal (r=0.9996), the average recovery rate was 95.35%, and RSD was 3.79%.The linear curve of tested vitamin B₂ concentration within the range of 0.24∼1.66 μg/mL was ideal (r=0.9999).The average recovery rate was 102.40%, and RSD was 2.80%.The linear curve of tested vitamin B₆ concentration within the range of 0.39∼2.71 μg/mL was ideal (r=0.9999).The average recovery rate was 94.66%, and RSD was 2.76%.The method was simple, fast and accurate.It can be commonly used to determine the contents of water-soluble vitamins in compound vitamini B and belladonnae oral solution

A review.Sodium-glucose linked transporter 2 (SGLT2) becomes a non-insulin based new target of the medication for diabetes mellitus.The SGLT2 inhibitors have become research focus in recent years.In this article, we briefly introduced the discovery and synthesis of several newly approved SGLT2 inhibitors including empagliflozin, ipragliflozin, luseogliflozin and tofogliflozin.

A review.Graphical synthetic routes to canagliflozin were reviewed.