This article describes about the regenerative medicine research using temperature-responsive cell culture equipment UpCell has a surface uniformly grafted with poly-N-isopropylacrylamide with a thickness of the order of nanometers, and confluent cells can be collected in a sheet form only by temperature treatment. The cell sheet engineering originated in Japan is a new tissue construction method that does not use scaffolding materials, and has been applied to various human clin. studies such as the esophagus, cornea, heart, periodontal, and cartilage.