The novelty of this work is comparison for sliver oxide nanoparticles (AgO NPs) preparation using the wild shrimp extract via hydrothermal method before and after with pulse laser ablation (PLA) technique.Two distinct methods were used to successfully prepare AgO NPs, which involved mixing a wild shrimp extract with Ag2NO3 salt.The objective of this study is to identify a more appropriate method for the new morphol. of AgO NPs by comparing different methods, with the aim of achieving stable and size-controlled AgO NPs.The observation techniques employed included XRD, FE-SEM, UV-visible, PL spectroscopy, zeta potential anal., and DLS anal.AgO NPs created via hydrothermal method without the use of PLA had a small crystallite size (38.4 nm) with a cubic structure, according to XRD data; in contrast, AgO NPs prepared with PLA had a smaller crystallite size (9.4 nm), and the diffraction peaks showed a cubic structure with high crystal quality.FE-SEM micrograph showed that AgO NPs produced hydrothermally without PLA had particles ranging from 15 to 57 nm and a spherical shape, whereas those produced with PLA had lower crystallite sizes ranging from 15 to 31 nm.UV-vis observations revealed a blue shift in energy gap values, from 4.2 eV for AgO NPs using the hydrothermal method without the PLA technique to 4.25 eV for AgO NPs using the PLA technique.PL spectroscopy showed that AgO NPs without PLA emitted around 3.7 eV, but those with PLA emitted more than 5.5 eV.The hydrothermal preparation of AgO NPs resulted in inhibition zones measuring 32.5 mm for gram-pos. bacteria (Staphylococcus aureus) without using PLA, while using the PLA method yielded an inhibition zone of 29.5 mm.The zeta potential value of AgO NPs by the hydrothermal method without PLA is -16.14 mV, whereas the zeta potential value of AgO NPs using the PLA technique is -18.19 mV.To the best of the author′s knowledge, comparison between AgO NPs preparation using mixing the wild shrimp extract and Ag2NO3 salt via hydrothermal without and with PLA technique is a novel concept that has not been documented in any research papers.