Gansu Cheezheng Tibetan Medicine Co. Ltd.

Objective: To study the accelerated stability of Hydroxy safflor yellow A in Carthami Flos and provide a reasonable experiment basis for Carthami Flos and its related preparation storage.Methods: Hydroxy safflor yellow A in Carthami Flos was determined by high performance liquid chromatog., which was exposed to different temperature and humidity.Results: Temperature and humidity are related to the stability of Hydroxy safflor yellow A.Conclusion: Under lower temperature and humidity condition, Hydroxy safflor yellow A has a good stability.

The antibacterial components in the roots of Inula racemosa were isolated by various chromatog. methods and elucidated on the basis of spectroscopic data.And their antibacterial activities for Escherichia coli and Staphylococcus aureus were tested.Twelve components were obtained from EtOAc fraction in the ethanol extract from the roots of I. racemosa and identified as dehydrocostus lactone (1), 1α,5αH-13-hydroxyguaia-4(15),10(14),7(11)-trien-6α,12-olide (2), 1α,5α,7α,11H-guaia-4(15),10(14)-dien-6α,12-olide (3), 1α,5α,7αH-3β-hydroxyguaia-4(15),10(14),11(13)-trien-6α,12-olide (4), 1α,5α,7α,11βH-3β-hydroxyguaia-4(15),10(14)-dien-6α,12-olide (5), germacra-1(10),4(5),11(13)-trien-6α,12-olide (6), (E)-3-(4-hydroxyphenyl)-propenoic acid (7), 7-hydroxycoumarin (8), vanillin (9), 3β-hydroxy-5α,8α-epidioxyergosta-6,22-diene (10), β-sitosterol (11), and 1α,5αH-2α,6α-bis-(4-hydroxy-3,5-dihydroxyben)-3,7-dioxabicyclo[3.3.0]-octane (12).Compounds 2-5, 10 and 12 were obtained from the roots of I. racemosa for the first time.Antibacterial activity results of all the compounds showed that compounds 1, 4 and 6 had strong antibacterial activity.

The method of quality control of curcumin in Shibaweiheziliniao pills by RP-HPLC was established.A C₁₈ column was used with a mobile phase consisted of mixture of acetonitrile-4% glacial acetic acid (48:52) at flow rate of 0.8 mL/min^-1.And the UV detector wavelength was 430 nm.The linear range of curcumin was 0.01-0.20 μg (r = 0.9999).The average recovery was 99.08% (RSD = 1.44%, n = 6).This method is accurate, simple and rapid, and can be used in quality control of Shibaweiheziliniao pills.