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Baclofen/Naltrexone Hydrochloride/D-Sorbitol

巴氯芬/盐酸纳曲酮/山梨糖醇

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GABAB receptor x Opioid receptors x mAChRs

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GABAB receptor调节剂 [+2]

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最高研发阶段临床3期

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0 项与 Genenet Co. Ltd. 相关的专利（医药）

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项与 Genenet Co. Ltd. 相关的文献（医药）

2014-08-01·Archives of Oral Biology4区 · 医学

Inhibitory effects of ameloblastin on epithelial cell proliferation

4区 · 医学

Article

作者: Nishihara, Tatsuji ; Kamegawa, Mariko ; Saito, Noriko ; Akebiyama, Yasuo ; Ariyoshi, Wataru ; Matsukizono, Miho ; Kitamura, Chiaki ; Okinaga, Toshinori

OBJECTIVEAmeloblastin is an enamel matrix protein expressed in several tissues. Many potential mechanisms have been identified by which ameloblastin functions as an extracellular matrix protein. However, the biological effects of ameloblastin on gingival epithelial cells remain unclear. In the present study, we established a novel system to purify recombinant human ameloblastin and clarified its biological functions in epithelial cells in vitro.DESIGNRecombinant human ameloblastin was isolated from COS-7 cells overexpressing HaloTag-fused human ameloblastin by the HaloTag system and then purified further by reverse-phase high-performance liquid chromatography. SCC-25 cells, derived from human oral squamous cell carcinoma, were treated with recombinant ameloblastin and then cell survival was assessed by a WST-1 assay. Cell cycle analysis was performed by flow cytometry.RESULTSThe novel purification system allowed effective recovery of the recombinant ameloblastin proteins at a high purity. Recombinant ameloblastin protein was found to suppress the proliferation of SCC-25 cells. Flow cytometric analysis showed that ameloblastin treatment induced cell cycle arrest G1 phase.CONCLUSIONSWe developed a procedure for production of highly purified recombinant human ameloblastin. Biological analyses suggest that ameloblastin induces cell cycle arrest in epithelial cells and regulates the progression of periodontitis.

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100 项与 Genenet Co. Ltd. 相关的转化医学

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