A simple, precise and accurate RP-UPLC method has been developed and validated for dissolution testing of dutasteride in soft gelatin capsules. The quantification was carried out using a Phenomenex Kinetex C 18 (4.6 x 100 mm, 2.6 μm) column, with mobile phase A (potassium dihydrogen phosphate buffer solution, pH 3) and mobile phase B (Acetonitrile :Water [80:20]) were run isocratically at 30:70 ratio resp., at a flow rate of 0.8 mL/min and UV detection at 210 nm. The method was validated for specificity, stability of anal. solution, filter variability, linearity, accuracy, range, precision, intermediate precision, system suitability and robustness. The proposed method was successfully applied for dissolution testing of dutasteride from soft gelatin capsules.