Sichuan Agricultural University

The aim of this study was to detect the expression of GSK3B gene in different tissues of goats and the expression level of i.m. precursor adipocytes at different differentiation periods, and to further reveal the effects of overexpression and interferences of GSK3B gene on lipid deposition in i.m. precursor adipocytes of goats.The results showed that a nucleotide sequence of 1,433 bp, CDS region 1,263 bp, encoding 420 amino acids, was obtained for the goat GSK3B gene, Goat GSK3B gene expression was highest in the lungs, lowest in the longest dorsal muscle, and highest at 120 h of induced differentiation.Overexpression of GSK3B gene significantly reduced lipid droplets in i.m. precursor adipocytes, and also significantly down-regulated the relative expression of SREBP1, CEBPβ and PPARγ (p < 0.05), with no significant changes in the expression of FASN, CEBPα, SCD and LPL (p > 0.05), GSK3B inhibition increased lipid droplets accumulation by significantly down-regulated SREBP1, CEBPβ and PPARγ expression (p < 0.05).In conclusion, GSK3B expression significantly inhibited lipid deposition in goat i.m. precursor adipocytes.These results provide important data to further elucidate the mol. mechanisms regulating i.m. fat formation in goats.

The ketogenic diet (KD) induces prolonged hyperketonemia, characterized by elevated circulating level of β-hydroxybutyrate. However, the KD can negatively affect host metabolic health by altering the gut microbial community. Despite this, the regulatory effect of the gut microbiota on hepatic ketogenesis and triacylglycerol (TAG) accumulation during a KD remains poorly understood. Here, we hypothesized that the commensal bacterium regulates hepatic lipid metabolism in association with KD-induced hyperketonemia. The KD disrupts the remodeling of the gut microbiota following antibiotic-induced depletion. The capacity for ketogenesis and the severity of TAG accumulation in the liver closely correlated with changes in the gut microbial composition and the up-regulation of hepatic farnesoid X receptor (FXR), peroxisome proliferator-activated receptor alpha (PPARα), and diacylglycerol O-acyltransferase 2 (DGAT2), which were modulated by bile acid metabolism through the gut-liver axis. The commensal bacterium Clostridium perfringens type A is particularly implicated in prolonged hyperketonemia, exacerbating hepatic ketogenesis and steatosis by disrupting secondary bile acid metabolism. The increased conversion of deoxycholic acid to 12-ketolithocholic acid represents a critical microbial pathway during C. perfringens colonization. These findings illuminate the adverse effects of the gut microbiota on hepatic adaptation to a KD and highlight the regulatory role of C. perfringens in ketonic states.

Clin. mastitis in dairy cows directly affects the content and quality of milk, seriously restricting the healthy development of the dairy farming industry.It is very important to find efficient treatment methods with few side effects.AB4 has a good therapeutic effect on cows with clin. mastitis, but there has been no research on the mechanism of AB4 on the body or in the treatment of diseases based on proteomics technol.This experiment is based on Labelfree relative quant. proteomics technol. to screen the differential proteins of AB4 in the treatment of clin. mastitis and perform bioinformatics anal. to explore the protein regulatory role of AB4 in the treatment of mastitis and reveal the intrinsic role of AB4 in the treatment of mastitis. mechanism.