At present, the extraction of collagen is mainly from animal skin tissues, such as pigs, cattle, fish, and so on, which has certain virus hidden trouble.In order to obtain safer and better collagen, scholars have used gene recombination technol. to produce recombinant human collagen.Type III collagen is the main collagen in human skin, so the use of recombinant type III collagen is of great significance for the study of skin properties.The recombinant humanized type III collagen is obtained by optimizing recombinant expression of a human collagen original gene sequence that is highly consistent with a human natural collagen amino acid sequence.In order to explore the effect of recombinant humanized type III collagen on alleviating skin wrinkles when applied to cosmetics, its anti-wrinkle effect was verified at the skin functional gene level by testing on human skin fibroblasts and keratinocytes.The results show that 5 mg/mL RHC III can significantly promote the expression of collagen synthesis related genes Collagen I, Collagen III and Smad3 in human skin fibroblasts.Meanwhile, under UVA stimulation, the expression of collagen degradation related genes MMP-1, MMP-3, c-jun and c-fos can be significantly inhibited.Acting on keratinocytes can significantly promote the expression of skin barrier and moisturizing-related genes LOR, TGM1, IVL, OCLN1, DSG1, FLG, Caspase14 and K1.To sum up, the sample recombinant type III collagen has anti-wrinkle effect at the mass concentration of 5 mg/mL.