Dehydroepiandrosterone ( DHEA) role in enhancement and maintenance of implantation (DREAM) - Randomized Double Blind Placebo Controlled Trial - DREAM TRIAL

开始日期2020-06-29

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Craft Hospital & Research Centre

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2022-01-01·Journal of Human Reproductive Sciences

Mitochondrial DNA Levels in Trophectodermal Cells Show No Association with Blastocyst Development and Pregnancy Outcomes

Article

作者: Ashraf, Mohammed C ; Veerasigamani, Geetha ; Modi, Deepak ; Singh, Sankalp ; Laheri, Saniya ; Colaco, Stacy ; Ritu, G

ABSTRACTBackground:In patients undergoing assisted reproduction, levels of mitochondrial DNA (mtDNA) in the trophectodermal cells of the developing blastocyst are suggested to be associated with its ability to implant. However, discrepancies exist regarding the use of mtDNA levels as a reliable biomarker to predict outcomes of assisted reproduction.Aims:The aim of the study is to explore the association of trophectodermal mtDNA levels to determine blastocyst quality, implantation potential of blastocyst and clinical outcomes in couples who have undergone pre-implantation genetic testing for aneuploidy (PGT-A).Study Setting:Private fertility centre.Study Design:Retrospective analysis.Materials and Methods:We analysed mtDNA levels in the trophectodermal cells of 287 blastocysts from 61 couples undergoing PGT-A. The levels of mtDNA were estimated by next-generation sequencing method. mtDNA levels were correlated with maternal age, blastocyst morphology, ploidy status, implantation rates, miscarriage rate and live birth rate.Statistical Analysis Used:Linear regression and one-way ANOVA with Tukey's all column comparison test.Results:The trophectodermal mtDNA levels did not correlate with maternal age. There were no significant differences in their levels in grade 1 and grade 2 blastocysts. No significant differences were seen between mtDNA levels of implanted and non-implanted blastocysts or those blastocysts that resulted in miscarriage or live birth. However, significantly lower amounts of mtDNA were seen in euploid blastocysts as compared to that in aneuploid blastocysts.Conclusion:mtDNA levels in the trophectodermal cells of the blastocyst do not associate with blastocyst quality (grade 1 and grade 2), implantation potential and clinical outcomes but can differentiate between aneuploid and euploid blastocysts. Our study does not support the use of trophectodermal mtDNA levels as a biomarker for blastocyst quality and predictor of clinical outcomes.

2021-12-01·Journal of Genetic Engineering and Biotechnology

Encapsulated VEGF121-PLA microparticles promote angiogenesis in human endometrium stromal cells

Article

作者: Majiyd, Noushin Abdul ; Abraham, Sunil ; Chinnaiah, Amutha ; Sanjay, Geetha

AbstractBackgroundIn this study, Vascular Endothelial Growth Factor 121 expressed abundantly in endometrial stromal cells is encapsulated with poly-l-lactide and characterized the properties for endometrial angiogenesis. We studied the migration, proliferation and the protein levels of human immortalized endometrium stromal cells after treating the cells with recombinant Vascular Endothelial Growth Factor (200 and 500 nanogram), and poly-l-lactide loaded Vascular Endothelial Growth Factor 121 (day 1, 20 and 30). The present study explains endometrium angiogenesis because endometrium plays an important role in pregnancy.ResultsMigration and proliferation studies in endometrium cells proved the efficiency of Vascular Endothelial Growth Factor and poly-l-lactide loaded Vascular Endothelial Growth Factor 121. This proliferated and increased the migration of the cells in vitro and also activated the Protein kinase B, Phosphatidylinositol-4, 5-Bisphosphate 3-Kinase Catalytic Subunit Beta, α-Smooth muscle actin and vascular endothelial growth factor receptor 2 pathways. Western blot analysis showed the increased expression levels of kinases, smooth muscle actin and vascular endothelial growth factor receptor 2 after the treatment with Vascular Endothelial Growth Factor and poly-l-lactide loaded Vascular Endothelial Growth Factor 121 particles in comparison to the control group. The elevated levels of α-Smooth muscle actin in endometrium cells with Vascular Endothelial Growth Factor prove the regulation of angiogenesis in vitro.ConclusionEndometrium thickness is one of the important factors during implantation of embryo and pregnancy. Slow release of VEGF from PLA encapsulated microparticle further controls the endothelial cell proliferation and migration and helps in the promotion of angiogenesis. The combined effect studied in vitro could be used as a pro-angiogenic drug on further in vivo confirmation.

2021-11-01·Journal of Human Reproductive Sciences

Indian Society for Assisted Reproduction Consensus Guidelines on Preimplantation Genetic Testing in In vitro Fertilization Clinics

Article

作者: Selvaraj, Priya ; Mangoli, Vijay S ; Sethi, Feseena ; Chimote, Nishad ; Singh, Sarabpreet ; Chaitanya, Krishna ; Sharma, Dayanidhi ; Hari, Ritu ; Kandari, Sayali ; Gopinath, Parasuram ; Mehta, Manjeet S ; Malhotra, Keshav ; Chelur, Vijayakumar ; Malhotra, Jaideep ; Majumdar, Gaurav

Study Question:What are the good practice guidelines for Pre implantation genetic testing applicable in INDIA?What is Already Known:Pre-Implantation Genetic Testing (PGT) is not new in India. It is used to identify euploid embryos for transfer, thus enabling couples to achieve a healthy pregnancy. There has been a lot of controversy surrounding PGT in the international forums; most of these debates have failed to reach a consensus on whether PGT should be offered or its concerns be validated more.Study Design, Size, Duration:This is the report of a 2-day consensus meeting where two moderators were assigned to a group of experts to collate information on Pre implantation genetic testing and embryo biopsy practices in INDIA. This meeting utilised surveys, available scientific evidence and personal laboratory experience into various presentations by experts on pre-decided specific topics.Participants/Materials, Setting, Methods:Expert professionals from ISAR representing clinical, embryological and genetic fields.Main Results and the Role of Chance:The report is divided into various components defining the terminologies, the various requirements, qualifications, recommendations on PGT -A,M,SR, and quality management: the report and recommendations of the expert panel reflect the discussion on each of the topics and try to lay down good practice points for labs to follow.Limitations, Reasons for Caution:The recommendations are solely based on expert opinion. Future availability of data may warrant an update of the same.Wider Implications of the Findings:These guidelines can help labs across the country to standardise their PGT services and improve clinical outcomes.Study Funding/Competing Interest(S):The consensus meeting and writing of the paper was supported by funds from CooperSurgical India.

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