The method of determination of the glycyrrhizic acid content in Weitongning tablets with high performance liquid chromatograph method was established. Glycyrrhizic acid was extracted with mobile phase ultrasonic technique, with Kromasil ODS chromatog. column of 5 μm and 4.6 mm × 250 mm, and was separated with acetonitrile-0.01 mol/L phosphoric acid solution (38:62), with a flow rate of 1 mL/min and a wavelength of 250 nm. This method was convenient and accurate with good reproducibility, a concentration linear range of 0.1129-0.2635 mg/mL (r = 0.9997), an average recovery rate of 99.0% and relative standard deviation (RSD) of 1.3%. The established method can be used for the quality control of Weitongning tablets.