Malian saddle 75% ethanol extract of the root macroporous resin column 60% ethanol elution the chem. constituents was studied.Used such as silica gel column chromatog., ODS column chromatog. and preparative high performance liquid separated means to sep. the chem. components, and then by NMR determined its chem. structure.The isolated three white powder compounds; NMR chart determine if it was a cardiac glycosides.The three compounds were 6-O-acetylperiplogenin (1), periplogenin 3-O-β-D-digitoxoside (2), digitoxigenin gentiobioside (3), where in the compound 1 was a species within the first minute.The method malian chem. constituents of the saddle has a good application value and can be applied to the further development of Malian saddle.