The aim of this study was to establish an HPLC method for simultaneous determination of the content of four flavonoid glycosides in Shanlamei Qinggan Cha. Using an Elite hypersil ODS2 column (4.6 mm×250 mm, 5 μm), the mobile phase was acetonitrile-0.1% acetic acid, with flow rate of 1.0 mL·min-1. The detective wavelength was at 344 nm and the column temperature was 35°C. The standard curve presented a linear range from 7.012-70.12 mg·L-1 for rutin (r=0.9996), the average recovery was 100.68% with RSD of 3.27%. The calibration curve was linear in the range of 5.126-51.26 mg·L-1 for kaempferol-3-O-β-D-galactose-(6-1)-α-L-rhamnoside (r=0.999 9), the average recovery was 98.48% with RSD of 1.7%. The calibration curve was linear in the range of 2.631-26.31 mg·L-1 for kaempferol-3-O-rutinoside (r=0.9998), the average recovery was 101.82% with RSD of 1.9%. The standard curve presented a linear range from 2.634-26.34 mg·L-1 for astragalin (r=0.9999), the average recovery was 97.02% with RSD of 1.7%. The method was fast, accurate and simple in good reproducibility, which could be used to control the quality of Shanlamei Qinggan Cha.