Supramolecular dye self-assembly mediated by host-guest interactions for the sensitive and portable detection of pyrene derivatives in environmental samples and urine

Article

作者: Wu, Chengqiu ; Yu, Bei ; Ding, Chunguang ; He, Yazhen ; Zheng, Yuqiao ; Meng, Jing ; Yang, Yina ; Tao, Yucun ; Zhu, Wenxuan ; Peng, Fangda ; Meng, Qingyu ; Wang, Chaoyang ; Yu, Lijia

Polycyclic aromatic hydrocarbons (PAHs), especially pyrene, are hazardous pollutants with serious health risks. Effective detection methods for PAHs are essential for environmental monitoring. In this study, we construct a simple, efficient method to detect pyrene derivatives in water. A squaraine dye (J3-Ad) with dual host-guest sites was synthesized and paired with a β-cyclodextrin dimer (H2-CD) to regulate host-guest interactions. In the presence of pyrenes, J3-Ad monomers in the J3-Ad/H2-CD mixture (JH-AC) are displaced by pyrenes and self-assemble into H-aggregates, resulting in a ∼135 nm absorption spectral shift. The transformation was confirmed through Scanning Electron Microscope (SEM), Dynamic Light Scattering (DLS), and Density Functional Theory (DFT) analysis. The system showed high sensitivity, with detection limits of 1.76 nmol/L for pyrene and 60.02 nmol/L for 1-hydroxypyrene (1-OHP), along with strong anti-interference and reliable colorimetric recognition. A smartphone-based, real-time detection platform was developed for visual monitoring of pyrene in soil and vegetables. Pyrene in tap water and river water, as well as 1-OHP in urine, were successfully detected, with acceptable recovery rates and a relative standard deviation (RSD) of less than 10.14 %. This work provides a sensitive, rapid, and visual method for tracking PAH pollution, offering significant potential for practical, on-site environmental applications.

2025-03-01·Analytical Sciences

Squaraine dye as a fluorescent probe for highly sensitive detection of pyrophosphate and alkaline phosphatase

Article

作者: Zhu, Wenxuan ; Yu, Lijia ; Zhang, Xiufeng ; Wu, Chengqiu ; Tao, Yucun ; Meng, Jing ; Yu, Bei ; Shi, Lei ; Ding, Chunguang ; Zhao, Shuhua ; Wang, Chaoyang

We synthesized a squaraine dye (F-0) to develop a method for detecting pyrophosphate (PPi) and alkaline phosphatase (ALP) by modulating the fluorescence of F-0. The fluorescence intensity of the F-0 system was quenched upon the addition of Cu²⁺ ions; however, it was restored when PPi was introduced due to the formation of a complex between PPi and Cu²⁺. Since ALP can hydrolyze PPi, the fluorescence of the system was quenched again upon the addition of ALP. Based on these principles, we established a fluorescent probe that exhibits an "off-on-off" fluorescence response. The detection limits of this method for PPi and ALP were 103 nmol dm^-3 and 0.18 U dm^-3, respectively. Moreover, this method demonstrates good selectivity and specificity and can be applied to the detection of PPi in actual samples.

2025-01-01·Leukemia Research

The increase in the expression of circRNAs may contributes to a poor prognosis in acute myeloid leukemia: A systematic review and meta-analysis

Review

作者: Yu, Yuandong ; Xiao, Yue ; Zhang, Shiming ; Li, Shen ; Zhang, Bohao ; Song, Ruipeng ; Liu, Mengfei ; Wei, Junfan ; Li, Meng

OBJECTIVEThe primary methods for defining the prognostic risk of AML patients are cytogenetic and molecular analysis at the time of diagnosis. However, the prognosis of intermediate-risk patients is still not well assessed for biomarkers. The main objective of this meta-analysis is to evaluate the relationship between circRNAs and AML prognosis, to provide a theoretical basis for finding effective prognostic indicators in intermediate-risk patients, and to provide an important scientific basis for the development or revision of WHO practice guidelines and ELN risk classification, and to highlight the importance of continuing to focus on and evaluate the prognostic impact of circRNAs on AML in future studies.METHODSWe performed a comprehensive literature search across PubMed, the Cochrane Library, and Web of Science databases for studies published up to September 15, 2024. Articles were selected based on inclusion criteria. The Newcastle-Ottawa Scale (NOS) was used to evaluate the quality of studies. The outcome measure of overall survival (OS) was used, and hazard ratios (HR) and 95 % confidence intervals (CI) were pooled to estimate the relationship between circRNA expression and prognosis in AML using STATA 17.0 software.RESULTSA total of 13 studies involving 1401 AML patients were included. The studies showed a significantly increased hazard ratio (HR) of upregulated CircRNA expression for OS (HR=1.87, 95 % CI=1.51-2.32, P < 0.001). The results of subgroups analysis showed a significant increase in the hazard ratio (HR) for upregulation of CircRNA expression in EFS and circ_0012152（HR= 1.66, 95 % CI= 1.19-2.32, P < 0.005 and HR= 2.26,95 % CI= 1.27-4.00, P < 0.005), respectively. No significant heterogeneity or publication bias was detected.CONCLUSIONUpregulated circRNA expression is significantly associated with poor prognosis in AML patients and may serve as a prognostic marker for AML.

100 项与 The Third People'S Hospital of Henan Province 相关的药物交易

登录后查看更多信息

100 项与 The Third People'S Hospital of Henan Province 相关的转化医学

登录后查看更多信息