A Randomized, Open and Routine Parallel Controlled Clinical Study on the Safety and Efficacy of Autologous Bone Marrow Mesenchymal Stem Cells (BMSCs) Transplantation in the Treatment of Ischemic Stroke

This study is to evaluated the safety and efficacy of BMSCs transplantation in the treatment of ischemic stroke, so as to provide a basis for future clinical application of BMSCs transplantation in the treatment of ischemic stroke.

开始日期2022-02-23

申办/合作机构

南方医科大学珠江医院

JPRN-JMA-IIA00329

/ Recruiting临床2期IIT

Intravenous infusion of autologous mesenchymal stem cells from bone marrow for chronic spinal cord injury patients (Phase II)

开始日期2018-08-13

申办/合作机构-

JPRN-JMA-IIA00330

/ Recruiting临床2期IIT

Intravenous infusion of autologous mesenchymal stem cells from bone marrow for chronic stroke patients (Phase II)

开始日期2018-07-30

申办/合作机构-

100 项与 Autologous Bone Marrow Mesenchymal Stem Cells(Zhujiang Hospital) 相关的临床结果

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100 项与 Autologous Bone Marrow Mesenchymal Stem Cells(Zhujiang Hospital) 相关的转化医学

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100 项与 Autologous Bone Marrow Mesenchymal Stem Cells(Zhujiang Hospital) 相关的专利（医药）

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406

项与 Autologous Bone Marrow Mesenchymal Stem Cells(Zhujiang Hospital) 相关的文献（医药）

2025-08-01·Tissue and Cell

Icariside II enhances crania defect repair through synergistic angiogenesis and osteogenesis

Article

作者: Yao, Zhangshun ; Luo, Guangming ; Zhang, Jing ; Zou, Leiyan ; Huang, Weixiang ; Jiang, Jingxian ; Zhang, Yunpeng

Vascularized bone tissue engineering for osteogenesis is considered a key approach for the repair of critical bone defects. Icariin(ICA) has been employed in bone tissue engineering for osteogenesis in several studies, demonstrating significant angiogenic and osteogenic effects in vivo in rat models. However, the in vivo angiogenic and osteogenic effects of Icariside II (ICSII), a gastrointestinal metabolite of ICA, remain unclear. Our preliminary study indicated that ICSII upregulated the expression of angiogenic and osteogenic differentiation markers in rat Bone Marrow Mesenchymal Stem Cells(rBMSCs) in vitro. Consequently, we loaded ICA and ICSII onto rBMSCs on Calcium Phosphate Cement(CPC) to construct the complexes CPC/ICA/BMSCs and CPC/ICSII/BMSCs, respectively. Scanning electron microscopy (SEM) revealed that BMSCs exhibited a more favorable morphology for proliferation, adhesion, and spreading on the scaffolds containing ICA and ICSII. Additionally, CPC/ICSII/BMSCs were implanted into a rat bilateral 5-mm critical skull defects model. Micro-CT analysis revealed that the CPC/ICA/BMSCs and CPC/ICSII/BMSCs groups exhibited the most significant promotion of new bone formation and neovascularization. Notably, the CPC/ICSII/BMSCs group demonstrated a significantly stronger neovascularization response than the CPC/ICA/BMSCs group. Histomorphometric analysis further revealed significant collagen fiber, new bone, and neovascularization formation in the CPC/ICA/BMSCs and CPC/ICSII/BMSCs groups, compared to the other groups. Thus, this study concludes that CPC/ICSII/BMSCs, which possess dual osteogenic and angiogenic properties, hold significant potential for repairing critical-size bone defects.

2025-05-01·Journal of Surgical Research

Bone Marrow-Derived Mesenchymal Stem Cells Alleviate Posthemorrhagic Shock Mesenteric Lymph-Induced Acute Lung Injury

Article

作者: Li, Zhonghua ; Wang, Wendi ; Du, Huibo ; Zhao, Zhen-Ao ; Wu, Xiaohui ; Zhao, Zi-Gang ; Suo, Tingjiao ; Niu, Chun-Yu

INTRODUCTIONMesenteric lymph is recognized as a conduit in the gut-lung axis. Posthemorrhagic shock mesenteric lymph (PHSML) contains proinflammatory substances and can exacerbate the acute lung injury (ALI) induced by hemorrhagic shock (HS). Mesenchymal stem cells (MSCs) possess anti-inflammatory properties and hold therapeutic potential for ALI. However, the effect and mechanism of MSCs in alleviating PHSML-mediated ALI remains unclear.METHODSRat hemorrhage shock model and PHSML infusion model were used to induce ALI. MSCs were administrated intravenously to treat ALI. Pulmonary function of rats was assessed by a Buxco pulmonary function analysis system. Hematoxylin and eosin staining was used for histological analysis. Western blot and quantitative real-time polymerase chain reaction were used to detect the expressions of inflammation-related genes.RESULTSIntravenous infusion of bone marrow-derived MSCs (BMSCs) prolonged the survival of HS rats. Both HS and PHSML could cause pulmonary tissue damage, lung edema, and pulmonary dysfunction, which were all alleviated by BMSC treatment. The pulmonary dysfunction indices (inspiratory resistance, functional residual capacity, and mean mid expiratory flow) were significantly improved by BMSC treatment in the two models. C-X-C motif chemokine ligand and inducible nitric oxide synthase, which are important for neutrophil recruitment and infiltration to the injured site, were down-regulated by BMSCs in the lung tissues of rats with HS or PHSML injury. As a neutrophil marker, myeloperoxidase is also decreased by BMSC treatment. These results indicated that BMSCs may reduce neutrophil recruitment and infiltration through inhibiting C-X-C motif chemokine ligand and inducible nitric oxide synthase expressions.CONCLUSIONSThe current findings demonstrate that BMSC therapy can alleviate the ALI induced by PHSML. In mechanism, BMSCs can protect lungs from the inflammatory response mediated by neutrophils. Our study provides novel insight to treat ALI in the gut lymphatics-lung axis.

2025-05-01·Brain Research Bulletin

Neuroprotective effects of bone marrow mesenchymal stem cells combined with mannitol on radiation-induced brain injury by regulating autophagy via the PI3K/AKT/mTOR signaling pathway

Article

作者: Song, Xue ; Liu, Zhanhong ; Zhang, Na ; Xu, Kaina ; Teng, Ye ; Pan, Shichao ; Wen, Yuxin ; Tong, Xu ; Tong, Xingbo

BACKGROUNDBone marrow mesenchymal stem cells (BMSCs) have demonstrated potential in the treatment of radiation-induced brain injury (RIBI); however, the presence of the blood-brain barrier (BBB) limits their therapeutic efficacy. Additionally, the precise mechanisms behind the use of BMSCs in treating RIBI are still not well understood. This study aimed to investigate the therapeutic efficacy of mannitol and BMSCs on neuronal autophagy and their efficacy in treating RIBI.METHODSIn the study, RIBI models were first established in male Sprague-Dawley (SD) rats. Evans blue staining was performed to examine how mannitol influences BBB permeability in RIBI. We isolated BMSCs from SD rats using the whole bone marrow adherent method and assessed their adipogenic and osteogenic differentiation potential through Oil Red O and Alizarin Red S staining; flow cytometry analysis assessed cell surface markers. Prussian blue staining was employed to verify the migration of iron-labeled BMSCs into brain tissue. The rats were then divided into specific treatment groups, and model establishment followed according to experimental conditions. The body weight of the rats was measured weekly throughout the study. Cognitive function was assessed using the Morris water maze test. H&E and Nissl staining were applied to evaluate hippocampal neuronal survival. We quantified key proteins in the PI3K/AKT/mTOR signaling pathway by Western blotting, and quantified autophagy-related proteins LC3B and beclin-1 using both Western blotting and immunofluorescence.RESULTSMannitol treatment significantly increased BBB permeability and promoted BMSCs migration. The combination of BMSCs and mannitol improved cognitive and memory functions, leading to better body weight recovery compared to the BMSCs group. H&E and Nissl staining also revealed a significant increase in neuronal survival within the combined treatment group. Furthermore, we observed through Western blot and immunofluorescence analyses that combination of BMSCs and mannitol enhanced the activation of PI3K, AKT, and mTOR through phosphorylation, while it reduced the expression levels of LC3B and beclin-1.CONCLUSIONThe combination of BMSCs and mannitol treatment significantly improved cognitive function and hippocampal neuronal survival in RIBI rats. This effect was achieved by increasing BBB permeability, facilitating BMSCs migration to the injured region, and regulating excessive autophagy through the PI3K/AKT/mTOR pathway. This combined treatment demonstrated a neuroprotective effect superior to that of BMSCs treatment alone.

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