A comprehensive chromatographic strategy for assessing the impact of artificial rearing, host tree species and environmental factors on quality of Cicadae Periostracum

Article

作者: Zhang, Huan-Huan ; Wu, Cheng-Ying ; Zhou, Jing ; Yue, Feng-Ru ; Xu, Jin-Di ; Kong, Ming ; Li, Song-Lin ; Mao, Qian ; Zhou, Shan-Shan ; Zhang, Min ; Shen, Ji-Chen ; Chen, Lin-Xia

Cicadae Periostracum (CP), derived from the slough of Cryptotympana atrata Fabricius, is a widely used insect-based medicinal material in traditional Chinese medicine. Due to the depletion of natural resources, artificial rearing has become prevalent; however, its impact on CP quality remains insufficiently elucidated. To evaluate this impact, along with that of host tree species and environmental factors, a comprehensive chromatographic strategy integrating UPLC-Q-TOF-MS/MS, UPLC-TQ-MS/MS and UPLC-DAD was developed for qualitative and quantitative analysis of N-acetyldopamine oligomers (NAOs), the active components in CP, as well as free/hydrolyzed amino acids (FAA/HAA). The extraction conditions for NAOs were optimized by response surface method. The results indicated that chemical profiles of CP obtained from wild collecting/artificial rearing, different host trees and regions exhibited a high degree of consistency. The artificial rearing CP samples showed higher levels of NAOs, FAA and HAA. Radar Plot Analysis showed that CP sourced from Malus pumila Mill (Mal) and Salix babylonica L. (Sal) achieved higher scores compared to five host trees studied. CP from Tengzhou region demonstrated relatively higher levels of NAOs and HAA. NAOs negatively correlated with annual near-surface air temperature, while HAA positively correlated with soil water content. These findings suggested that artificial rearing enhances CP quality, with Mal and Sal as promising host trees. Regions with lower annual near-surface air temperatures may promote NAOs accumulation. Overall, this research highlights the role of artificial rearing, host tree species and key environmental factors on the quality of CP and provides valuable insights for sustainable CP production and quality assessing.

2025-05-01·INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES

Effect of hydrophobically-modified sulfonated lignin on thermal stability of disperse dye paste

Article

作者: Zhou, Mingsong ; Pang, Yuxia ; Qian, Yong ; Wang, Gengbin ; Yang, Dongjie

This study aims to develop a lignin-based dispersant with excellent high-temperature dispersion properties by introducing cyclohexyl and phenyl hydrophobic groups into sulfomethylated lignin (SAL) molecules through a grafting modification strategy, thereby addressing the limitations of traditional disperse dye pastes in terms of grinding efficiency and thermal stability. The results show that graft-modified lignin dispersants outperform both SAL and the commercial dispersant Reax-85A. They not only improve the grinding efficiency of disperse dye pastes but also markedly enhance their thermal stability. The particle size of disperse dye paste significantly decreased from greater than 5 μm to 1 μm. The lignin dispersant containing cyclohexyl and phenyl groups significantly enhanced the hydrophobic van der Waals adsorption forces toward disperse dyes. Under high-temperature conditions, the prepared disperse dye pastes maintained excellent diffusion performance and thermal stability. QCM-D studies revealed that the adsorption capacity |ΔF| of hydrophobically-modified SAL on disperse dyes was significantly enhanced compared to that of SAL and Reax-85A, and it exhibited strong adsorption stability with minimal desorption, demonstrating robust adhesion to disperse dyes. Furthermore, the adsorption quantity of phenyl-modified SAL on disperse dyes was greater than that of cyclohexyl-modified SAL.

2025-04-01·ANNALS OF SURGERY

Graft Inflow Modulation by Splenic Artery Ligation for Portal Hyperperfusion Does Not Decrease Rates of Early Allograft Dysfunction in Adult Live Donor Liver Transplantation

Article

作者: Kumar, Anubhav ; Yadav, Anil ; Patil, Nilesh Sadashiv ; Mohapatra, Nihar ; Tharun, Gattu ; Thapar, Shalini ; Pamecha, Viniyendra ; Sindwani, Gaurav ; Dhingra, Udit

Objective::

The primary objective was to compare the rates of early allograft dysfunction (EAD) in patients undergoing elective adult live donor liver transplantation (ALDLT) with and without graft portal inflow modulation (GIM) for portal hyperperfusion. The secondary objectives were to compare time to normalization of bilirubin and International Normalized Ratio, day 14 ascitic output more than 1 L, small-for-size syndrome, intensive care unit/high dependency unit and total hospital stay, and 90-day morbidity and mortality.

Background::

GIM can prevent EAD in ALDLT patients with portal hyperperfusion.

Methods::

A single-center randomized trial with and without GIM for portal hyperperfusion by splenic artery ligation (SAL) in ALDLT was performed. After reperfusion, patients with portal venous pressure (PVP)>15 mm Hg with a gradient (PVP—central venous pressure) of ≥7 mm Hg and/or portal venous flow (PVF) >250 mL/min/100 g of liver were randomized into 2 groups: GIM and No GIM.

Results::

75 of 209 patients satisfied the inclusion criteria, and 38 underwent GIM. Baseline PVF and PVP were comparable between the GIM and no GIM groups. SAL significantly reduced the PVF and PVP (P<0.001). There were no significant differences in the primary and secondary outcomes between the 2 groups. In the subgroup analysis, with a Graft to Recipient Weight Ratio ≤0.8, there were no significant differences in the primary and secondary outcomes.

Conclusions::

SAL significantly decreased PVP and PVF but did not decrease rates of EAD in adult LDLT.

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