Selenium nanozyme-crosslinked composite hydrogel for promoting cartilage regeneration in osteoarthritis via an integrated ‘outside-in’ and ‘inside-out’ strategy

Article

作者: Jia, Guang ; Yuan, Guangfu ; Zhou, Guoqiang ; Gao, Wenyan ; Jin, Yi ; Li, Gaoyang ; Li, Shaochun ; Yang, Xinjian ; Miao, Ya

Osteoarthritis (OA), a degenerative joint disease, is characterized by chondrocyte senescence, extracellular matrix (ECM) degradation, and chronic inflammation, with limited regenerative capacity. Current therapies primarily provide symptom relief, therefore highlighting the need for more effective strategies to address OA's multifactorial pathology. This study introduces an innovative selenium nanozyme-crosslinked injectable composite hydrogel (Se/PRP-OGel), which combines selenium nanoparticles (SeNPs) with platelet-rich plasma (PRP) in a biocompatible oxidized chondroitin sulfate-gelatin scaffold (OGel), to address OA through an integrated "outside-in" and "inside-out" strategy. The "outside-in" strategy utilizes SeNPs to scavenge reactive oxygen species (ROS), alleviate oxidative stress, and restore redox balance, thereby reducing extracellular damage and modulating inflammation in the OA microenvironment. Concurrently, the "inside-out" strategy utilizes PRP's bioactive growth factors (e.g., TGF-β, IGF, FGF) to rejuvenate senescent chondrocytes, stimulate proliferation, and enhance ECM synthesis, creating a regenerative microenvironment. The results showed that Se/PRP-OGel demonstrated excellent biocompatibility, reduced ROS levels, mitigated chondrocyte senescence, and balanced ECM homeostasis. Moreover, it promoted cartilage repair, pain relief, and functional restoration in an OA rat model. This dual approach interrupts OA's degenerative cycle and fosters cartilage regeneration, providing a groundbreaking solution for effective cartilage regeneration and OA treatment.

2025-07-01·Domestic Animal Endocrinology

Effect of selenium and zinc nanoparticles supplementations on testicular blood flow, semen, and reproductive hormones in Egyptian native goats subjected to ambient heat stress

Article

作者: Aboelmaaty, Amal M ; Ragab, Refaat S A ; Saudi, Eltaher M ; NasrEldeen, Mohammed S

For exploring the protective effects of selenium (SeNPs) and zinc (ZnNP) nanoparticles on testicular blood flow, morphometry, oxidants-antioxidant status, reproductive hormones, and blood biochemicals of goats subjected to heat stress, bucks (n=15) were equally divided into control, ZnNP (50.0 mg/animal), and SeNPs (0.57 mg/animal) groups. Bucks were supplemented daily for seven weeks during summer. The testicular artery diameter, PSV, EDV, TAMV, Mean V, RI, PI, BFV, testicle length, width, height, epididymal length, and width were determined. Blood samples were collected for measuring LH, testosterone and estradiol, zinc, selenium, NO, MDA, SOD, TAC, CAT, total proteins, albumin, AST, and ALT. Results indicated that testicular volume, mediastinum testes, height, epididymal tail length, and width increased (P<0.0001) in animals supplemented with ZnNPs. Testicular (P<0.05) and epididymal tail (P<0.01) width increased during the supplementation. SeNPs and ZnNPs decreased (P<0.0001) the testicular artery diameter and increased PSV (P<0.05), and V Mean (P<0.01). ZnNPs increased TAMV (P<0.05) and BFV (P<0.01). RI (P<0.01), PI (P<0.05), and BFV (P<0.01) decreased after withdrawing supplementation associated with increased (P<0.05) testicular artery diameter. Sperm mass and individual motility, live sperm %, sperm cell concentration, and SPMI Improved (P<0.0001) in ZnNPs and SeNPs groups during and after the supplementation. ZnNPs and SeNPs decreased CAT (P<0.0001) and testosterone with increasing SOD (P<0.05), globulin, and ALT. SeNPs increased (P<0.0001) estradiol and LH. In conclusion, ZnNPs or SeNPs improved the semen parameters during heat stress by modulating testicular blood flow, testicular thermoregulatory mechanism, and antioxidant status, proving nontoxic to the liver and kidneys.

2025-07-01·Colloids and Surfaces B: Biointerfaces

Selenium nanoparticles as catalysts for nitric oxide generation

Article

作者: Boyer, Cyrille ; Chandrawati, Rona ; Mazur, Federico ; Fan, Qingqing ; Cheong, Soshan ; Geng, Shu ; Zhou, Yingzhu ; Ng, Gervase

The critical role of nitric oxide (NO), a potent signalling molecule, in various physiological processes has driven the development of NO delivery strategies for numerous therapeutic applications. However, NO's short half-life poses a significant challenge for its effective delivery. Glutathione peroxidase, a selenium-containing antioxidant enzyme, can catalyse the decomposition of S-nitrosothiols (endogenous NO prodrugs) to produce NO in situ. Inspired by this, we explored selenium nanoparticles (SeNPs) for their enzyme-mimicking NO-generating activity. Stabilised with polyvinyl alcohol (PVA) or chitosan (CTS), SeNPs demonstrated tuneable NO generation when exposed to varying concentrations of NO prodrug, nanoparticles, and glutathione (GSH). In the presence of GSH, a naturally occurring antioxidant in the human body, 0.1 µg mL^-1 of SeNPs could catalytically generate 7.5 µM of NO under physiological conditions within 30 min. We investigated the effects of nanoparticle crystallinity and NO prodrug type on NO generation, as well as the stability and sustained NO generation of the catalytic nanoparticles. PVA-stabilised SeNPs were non-toxic to NIH 3T3 cells and effectively dispersed Pseudomonas aeruginosa biofilms upon NO generation. This study broadens the repertoire of nanomaterials for NO generation and highlights SeNPs as a non-toxic alternative for therapeutic NO delivery.

100 项与 Selenium nanoparticles(SeNPs) 相关的药物交易

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适应症	最高研发状态	国家/地区	公司	日期
缺血性肾病	临床前	中国	广东医科大学附属医院	2024-07-25
再灌注损伤	临床前	中国	广东医科大学附属医院	2024-07-25
帕金森病	临床前	中国	华南理工大学	2024-07-01