The Effect of Bacillus Calmette-Guerin Polysaccharide Nucleic Acid (BCG-PSN) Acupoint Injection Combining Qingfei Sanjie Decoction on Small Pulmonary Nodules

开始日期2023-01-06

申办/合作机构

浙江中医药大学附属第三医院

ChiCTR2200060565 / SuspendedN/AIIT

Clinical Study of Qingfei Litan Decoction on Airway Mucus Hypersecretion in Stable Bronchiectasis

开始日期2022-05-30

申办/合作机构

中国中医科学院西苑医院

100 项与清肺汤相关的临床结果

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100 项与清肺汤相关的转化医学

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100 项与清肺汤相关的专利（医药）

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项与清肺汤相关的文献（医药）

2024-04-01·Journal of Ethnopharmacology

Guben Qingfei decoction attenuates LPS-induced acute lung injury by modulating the TLR4/NF-κB and Keap1/Nrf2 signaling pathways

Article

作者: Shi, Yuanyuan ; Zeng, Ziyuan ; Chen, Sheng ; Ding, Qi ; Fu, Yuchen ; Li, Minfang

ETHNOPHARMACOLOGICAL RELEVANCEAcute lung injury (ALI) is a life-threatening and widespread disease, with exceptionally high morbidity and mortality rates. Unfortunately, effective drugs for ALI treatment are currently lacking. Guben Qingfei decoction (GBQF) is a Chinese herbal compound known for its efficacy in treating viral pneumonia, yet the precise underlying mechanisms remain unknown.AIM OF THE STUDYThis study aimed to validate the mitigating effect of GBQF on ALI and to further investigate its mechanism.MATERIALS AND METHODSAn ALI mice model was established by infusing LPS into the endotracheal tube. The effects of GBQF on ALI were investigated by measuring lung W/D; MPO; BALF total protein concentration; total number of cells; TNF-α, IL-1β, and IL-6 levels; pathological changes in lung tissue, and oxidation products. Immunohistochemistry and Western Blotting were performed to verify the underlying mechanisms. MH-S and BEAS-2B cells were induced by LPS, and the effects of GBQF were confirmed by RT-PCR and immunofluorescence.RESULTSGBQF significantly reduced LPS-induced ALI in mice, improved lung inflammation, reduced the production of oxidative products, increased the activity of antioxidant enzymes, and reduced the degree of lung tissue damage. GBQF prevents MH-S cells from releasing inflammatory factors and reduces oxidative damage to BEAS-2B cells. In vivo studies have delved deeper into the mechanism of action of GBQF, revealing its correlation with the TLR4/NF-κB and Keap1/Nrf2 pathways.CONCLUSIONSOur study demonstrates that GBQF is an effective treatment for ALI, providing a new perspective on medication development for ALI treatment.

2024-01-01·Journal of Ethnopharmacology

Qingjie decoction attenuated E.coli-induced diarrhea by regulating energy metabolism and alleviating inflammation based on network analysis and metabolomics

Article

作者: Zhao, Qing-Yu ; Wang, Hui-Ru ; Zhang, Yan-Nan ; Fan, Yi-Meng ; Ga, Yu ; Hao, Zhi-Hui ; Wei, Yuan-Yuan

ETHNOPHARMACOLOGICAL RELEVANCEDiarrhea is a frequently encountered gastrointestinal complication in clinical practice, and E. coli is one of the main causative agents. Although Qingjie decoction (QJD) has been shown to be highly effective in treating diarrhea by eliminating heat-toxin, the underlying molecular mechanisms and pathways of QJD remain unclear.AIM OF REVIEWThe aim of this research was to explore the effects and fundamental mechanism of QJD on diarrhea induced by E.coli in rats.MATERIALS AND METHODSInitially, we used UHPLC-MS/MS analysis to identify the chemical composition of QJD. Then, we constructed a visualization network using network pharmacology. Next, we utilized metabolomics to identify differentially expressed metabolites of QJD that are effective in treating diarrhea.RESULTSThe chemical composition of QJD was analyzed using UHPLC-MS/MS, which identified a total of 292 components. Using a network pharmacology approach, 127 bioactive compounds of QJD were screened, targeting 171 potential diarrhea treatment targets. TNF-α, IL-6, IL-1β, and CAT were identified as important targets through visualizing the PPI network. Enrichment analysis demonstrated significant enrichment in the TNF signaling pathway, IL-17 signaling pathway, and PI3K-Akt signaling pathway. QJD showed beneficial effects, such as increased body weight, decreased fecal water content, and reduced inflammatory cell infiltration in the duodenum and colon, as well as maintaining the structure of the duodenum and colon. Metabolomic analysis revealed 32 differentially expressed metabolites in the control, model and QJD-H groups, including glucose, valine, and cysteine. Functional analysis indicated that differential metabolites were related to energy metabolism, including glucose metabolism, TCA cycle, and amino acid metabolism.CONCLUSIONQJD significantly increased body weight, decreased water content in feces, relieved inflammatory cell infiltration, maintained the structure of duodenum and colon. Combining network analysis and metabolomics, QJD exerted therapeutic effects by inhibiting inflammation and oxidative stress, regulating glucose metabolism, tricarboxylic acid metabolism, and amino acid metabolism.

2022-03-01·Journal of Zhejiang University (Medical Sciences)

Efficacy of Qingfei oral liquid for idiopathic pulmonary fibrosis in rats and related network pharmacology study

Article

作者: ZHANG, Yiwen ; SHENG, Kongsheng ; LIU, Yujia ; HUANG, Ping ; SONG, Feifeng ; ZOU, Xiaozhou ; PAN, Zongfu

To investigate the therapeutic effect and mechanism of Qingfei oral liquid in idiopathic pulmonary fibrosis. Seventy-two male SD rats were divided into control group, model group, pirofenidone group and Qingfei group with 18 animals in each group. The idiopathic pulmonary fibrosis was induced in last three groups by intratracheal injection of bleomycin; pirofenidone group was given oral administration of pirofenidone b.i.d for 21 d, and Qingfei group was given Qingfei oral liquid 3.6 mL/kg q.d for Lung tissues were obtained for HE staining, Masson staining and transforming growth factor (TGF)-β immunohistochemical staining. Superoxide dismutase (SOD), malondialdehyde (MDA) and glutathione (GSH) were detected in tissue homogenates. The BATMAN-TCM database was used to retrieve the chemical components and their corresponding targets of Qingfei oral solution by network pharmacology method, and then the component-target-disease network diagram was constructed. Finally, the pathway enrichment analysis was carried out to explore the molecular mechanism of Qingfei oral liquid against idiopathic fibrosis. Histopathology results showed that Qingfei oral liquid had a similar relieving effect on pulmonary fibrosis as the positive drug pirfenidone; TGF-β secretion had a significant reduction in lung tissues of Qingfei group; and Qingfei oral liquid had better regulatory effect on SOD, MDA and GSH than pirfenidone. The results of component-target-disease network and pathway enrichment analysis showed that the related molecular pathways were concentrated in inflammation, extracellular matrix and cytokines. Qingfei oral liquid has a good therapeutic effect on idiopathic pulmonary fibrosis in rats via regulation of inflammation, extracellular matrix and cytokines.

100 项与清肺汤相关的药物交易

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