Lanoteplase

In this study we have focused on three types of alcohols: ethanol (EtOH), 2-propanol (IPA), and 1-propanol (NPA), and examined the skin permeability of drugs with different physicochemical properties: ketoprofen (KPF; hydrophobic), cimetidine (CMT; slightly hydrophobic), and caffeine (CF; hydrophilic). The results revealed EtOH particularly enhanced the skin permeation of CF, while IPA enhanced skin permeation regardless of the type of drug. In contrast, NPA significantly increased the skin permeability of KPF and CMT, but had little effect on CF. The differing effects of the alcohols on skin permeation appear to be linked to the physicochemical properties of the drugs. KPF is more hydrophobic than the other drugs, suggesting that it uses the intercellular pathway in the stratum corneum for permeation. CMT has intermediate properties between hydrophilic and hydrophobic, resulting in low skin permeability and ineffective utilization of both the transepidermal and transappendageal pathways. CF mainly utilized the transappendageal pathways for skin permeation because of its smaller molecular weight and more hydrophilic as compared with the other drugs. These results suggest that the effect of different alcohols on enhancing drug skin permeation is not uniform and that the optimal alcohol for enhancing permeability may vary depending on the drug. Therefore, the selection of appropriate additives based on the physicochemical properties of the drug, such as hydrophilicity, hydrophobicity, and molecular weight, is crucial for developing effective transdermal formulation.

Polarized auxin transport regulates fruit shape determination by promoting anisotropic cell growth. Angiosperms produce organs with distinct shape resultant from adaptive evolution. Understanding the cellular basis underlying the development of plant organ has been a central topic in plant biology as it is key to unlock the mechanisms leading to the diversification of plants. Variations in the location of synthesis, polarized auxin transport (PAT) have been proposed to account for the development of diverse organ shapes, but the exact cellular mechanism has yet to be elucidated. The Capsella rubella develops a perfect heart-shaped fruit from an ovate shape gynoecium that is tightly linked to the localized auxin synthesis in the valve tips and provides a unique opportunity to address this question. In this study, we studied auxin movement in the fruits and the cellular effect of N-1-Naphthylphthalamic Acid (NPA) on the fruit shape determination by constructing the pCrPIN3:PIN3:GFP reporter and live-imaging. We found PAT in the valve epidermis is in congruent with fruit shape development and NPA treatment disrupts the heat-shaped fruit development mainly by repressing cell anisotropic growth with minor effect on division. As the Capsella fruit is unusually big in size, we also included a detailed step-by-step protocol on how to conduct live-imaging experiment. We further test the utility of this protocol by conducting a live-imaging analysis of the gynophore in Arachis hypogaea. Collectively, the results of this study elucidated the mechanism on how auxin signal was translated into instructions guiding cell growth during organ shape determination. In addition, the description of the detailed live-imaging protocol will encourage further studies of the cellular mechanisms underlying shape diversification in angiosperms.