Chloramine T

Free-living amoebae (FLA) are prevalent in drinking water distribution networks (DWDNs), yet our understanding of FLA community dynamics and assembly mechanisms in DWDNs remains limited. This study characterized the occurrence patterns of amoeba communities and identified key factors influencing their assembly across four full-scale DWDNs in three Chinese cities, each utilizing different disinfectants (chlorine, chloramine, and chlorine dioxide). High-throughput sequencing of full-length 18S rRNA genes revealed highly diverse FLA communities and an array of rare FLA species in DWDNs. Unique FLA community structures and higher gene copy numbers of three amoeba taxa of concern (Vermamoeba vermiformis, Acanthamoeba, and Naegleria fowleri) were observed in the chloraminated DWDN, highlighting the distinct impact of chloramine on shaping the amoeba community. The FLA communities in DWDNs were primarily driven by deterministic processes, with disinfectant and nitrogen compounds (nitrate, nitrite, and ammonia) identified as the main influencing factors. Machine learning models revealed high SHapley Additive exPlanations (SHAP) values of dominant amoeba genera (e.g., Vannella and Vermamoeba), indicating their critical ecological roles in shaping broader bacterial and eukaryotic communities. Correlation analyses between amoeba genera and bacterial taxa revealed that 82 % of the bacterial taxa exhibiting a negative correlation with amoebae were gram-negative, suggesting the preferred predation of amoebae toward gram-negative bacteria. Network analysis revealed the presence of only one to two amoebae in distinct modules, suggesting that individual amoebae might be selective in grazing. These findings provide insight into the amoeba community dynamics, assembly mechanisms and ecological roles of amoebae in drinking water, which can aid in risk assessments and mitigation strategies within DWDNs.

Seawater desalination is widely used to overcome the freshwater shortage worldwide. However, even after three-stage reverse osmosis treatment, the desalinated water still contained 14.6 μg/L of aliphatic disinfection byproducts (DBPs), 384.2 ng/L of bromophenolic DBPs, 3.5 ng/L of iodophenolic DBPs, 1024.7 μg/L of Br^-, 2.8 μg/L of I^-, and 2.4 mg C/L of dissolved organic carbon (DOC). After the desalinated water was disinfected with chlor(am)ine, the concentrations of halogenated aliphatic and phenolic DBPs further increased, and bromophenolic DBPs were the toxicity forcing agents. When surface water was mixed with desalinated water and then chlorinated, the yield of aliphatic and phenolic DBPs significantly elevated. Separately chlorinating desalinated water and surface water before mixing could mitigate this adverse situation. Chloramine disinfection was more conducive to reducing the total calculated toxicity of disinfected desalinated waters and mixed waters compared to chlorine disinfection. The treatment of desalinated water with granular activated carbon could effectively remove DOC and UV₂₅₄, leading to a reduction in the content of total organic halogen after chlor(am)ination. Although anion exchange resin could adsorb Br^-, it also released the organic precursors of DBPs, ultimately increasing the yield of DBPs. The results of this study can provide a reference for the seawater desalination industry to improve seawater pre-treatment and desalination processes and thus minimize the DBPs.