更新于：2024-09-19

DMRT1

更新于：2024-09-19

基本信息

别名

CT154、DM domain expressed in testis protein 1、DMRT1

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简介

Transcription factor that plays a key role in male sex determination and differentiation by controlling testis development and male germ cell proliferation. Plays a central role in spermatogonia by inhibiting meiosis in undifferentiated spermatogonia and promoting mitosis, leading to spermatogonial development and allowing abundant and continuous production of sperm. Acts both as a transcription repressor and activator: prevents meiosis by restricting retinoic acid (RA)-dependent transcription and repressing STRA8 expression and promotes spermatogonial development by activating spermatogonial differentiation genes, such as SOHLH1. Also plays a key role in postnatal sex maintenance by maintaining testis determination and preventing feminization: represses transcription of female promoting genes such as FOXL2 and activates male-specific genes. May act as a tumor suppressor. May also play a minor role in oogenesis (By similarity).

关联

100 项与 DMRT1 相关的临床结果

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100 项与 DMRT1 相关的转化医学

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0 项与 DMRT1 相关的专利（医药）

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项与 DMRT1 相关的文献（医药）

2025-01-01·Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology

miR-34 negatively regulates the expression of Dmrt and related genes in the testis of mud crab Scylla paramamosain

Article

作者: Wang, Yilei ; Zou, Pengfei ; Zhang, Ziping ; Jiang, Jing ; Huang, Yicong ; Wan, Haifu ; Jia, Xiwei ; Huang, Jinkun

The mud crab (Scylla paramamosain) is a commercially significant marine decapod crustacean. Due to its obvious sexual dimorphism, the mechanism of sex differentiation and gonadal development has attracted significant research interest. The Dmrt (double-sex and mab-3 related transcription factor) genes are vital in animal gonadal development and sex differentiation. In the present study, miR-34 was predicted to target the 3' end of Dmrt-1, idmrt-2, Dmrt-3, Dsx and Dmrt-like genes by prediction software, and the interactions between miR-34 and these Dmrt genes were validated by in vivo and in vitro experiments. Dual luciferase assay results indicated that miR-34 mimics/inhibitors co-transfected with plasmid vectors with 3' end of Dmrt-1, idmrt-2, Dmrt-3, Dsx and Dmrt-like, respectively, led to a significant decrease/increase of fluorescence activity in HEK293T cells. In vivo experiments showed that injection of agomir-34 significantly inhibited Dmrt-1, idmrt-2, Dsx and Dmrt-like expression, while injection of antagomir-34 caused the opposite result. However, Dmrt-3 expression was not affected by injection of miR-34 reagents. Meanwhile, the expression of spermatogenesis and testicular development-related molecular marker genes (IAG, foxl2 and vasa) in mud crabs was significantly changed after injecting the miR-34 reagent in vivo. Furthermore, the result of immunoblotting proved that the expression level of Dmrt-like protein can be regulated by miR-34. These results imply that miR-34 is indirectly involved in sex differentiation and testicular development of S. paramamosain by regulating Dmrt-1, idmrt-2, Dsx and Dmrt-like genes.

2024-12-01·Molecular Biology Reports

Roles of estrogen receptors during sexual reversal in Pelodiscus sinensis

Article

作者: Zhou, Tong ; Cao, Jizeng ; Chen, Guobin ; Liang, Hongwei ; Zou, Guiwei ; Wang, Long ; Li, Xiang ; Zhu, Chengjun

BACKGROUNDThe Chinese soft-shelled turtle, Pelodiscus sinensis, exhibits distinct sexual dimorphism, with the males growing faster and larger than the females. During breeding, all-male offspring can be obtained using 17β-estradiol (E2). However, the molecular mechanisms underlying E2-induced sexual reversal have not yet been elucidated. Previous studies have investigated the molecular sequence and expression characteristics of estrogen receptors (ERs).METHODS AND RESULTSIn this study, primary liver cells and embryos of P. sinensis were treated with ER agonists or inhibitors. Cell incubation experiments revealed that nuclear ERs (nERs) were the main pathway for the transmission of estrogen signals. Our results showed that ERα agonist (ERα-ag) upregulated the expression of Rspo1, whereas ERα inhibitor (ERα-Inh) downregulated its expression. The expression of Dmrt1 was enhanced after ERα-Inh + G-ag treatment, indicating that the regulation of male genes may not act through a single estrogen receptor, but a combination of ERs. In embryos, only the ERα-ag remarkably promoted the expression levels of Rspo1, Wnt4, and β-catenin, whereas the ERα-Inh had a suppressive effect. Additionally, Dmrt1, Amh, and Sox9 expression levels were downregulated after ERβ inhibitor (ERβ-Inh) treatment. GPER agonist (G-ag) has a significant promotion effect on Rspo1, Wnt4, and β-catenin, while the inhibitor G-Inh does not affect male-related genes.CONCLUSIONSOverall, these results suggest that ERs play different roles during sexual reversal in P. sinensis and ERα may be the main carrier of estrogen-induced sexual reversal in P. sinensis. Further studies need to be performed to analyze the mechanism of ER action.

2024-09-01·Biochimica et Biophysica Acta (BBA) - General Subjects

The Chinese medaka (Oryzias sinensis) dmrt1 gene converts females to males in medaka (Oryzias latipes)

Article

作者: Chen, Tian-Sheng ; Huang, Yan ; Chen, Lei ; Wang, Meng-Yang ; Liang, Jing-Jie ; Pan, Qi-Hua

BACKGROUNDChinese medaka (Oryzias sinensis) is widely distributed in freshwater rivers in China. Similar to the medaka (Oryzias latipes), Chinese medaka has the characteristics of small size, rapid reproductive cycle, and strong adaptability, which makes it suitable as a model organism for studies in basic biology and environmental toxicology. Chinese medaka exhibits distinct sexual dimorphism. However, due to the lack of complete genomic information, the regulation of sex determination and differentiation-related genes in Chinese medaka remains unclear.METHODSChinese medaka dmrt1 (Osdmrt1) was cloned by PCR, and transgenic individuals of medaka [Tg(CMV:Osdmrt1)] overexpressing Osdmrt1 were generated to investigate the role of Osdmrt1 in sex determination. Western blot was used to validate the integration of the Osdmrt1 into the medaka genome. Tissue sectioning and HE staining were used to identify Tg(CMV:Osdmrt1) physiological gender and phenotype. qRT-PCR was used to analyze the expression of gonad-specific genes.RESULTSOsdmrt1 was cloned and identified, and it shared similar evolutionary relationships with medaka dmrt1. Tg(CMV:Osdmrt1) exhibited partial sex reversal from female to male in the F2 generation, with genetically female individuals developing testes and producing functional sperm. Additionally, the secondary sexual characteristics of the transgenic females also changed to males.CONCLUSIONThe Chinese medaka dmrt1 gene could convert females to males in medaka.GENERAL SIGNIFICANCEThese results not only elucidate the function of Chinese medaka dmrt1, but also accumulate knowledge for studying the function of economically important fish genes in model fish by transgenic technology.

项与 DMRT1 相关的新闻（医药）

2023-08-30

·生物谷

曾发两篇Science论文，浙江万里学院葛楚天获国家杰青资助

该研究揭示了组蛋白去甲基化酶KDM6B调控红耳龟温度依赖型性别决定的分子机理，为“表观遗传机制调控温度依赖型性别决定”假说提供了首个直接遗传证据，解开了温度依赖型性别决定研究长达半个世纪的谜团。 2023年8月24日，国家自然科学基金委员会正式公布了2023年度国家自然科学基金集中接收申请项目的评审结果，浙江万里学院葛楚天教授申报的“水产动物繁殖与发育”项目获国家杰出青年科学基金项目资助，实现了浙江万里学院在国家杰出青年科学基金项目的历史性突破。据悉，水产领域获本年度国家杰出青年科学基金项目资助仅两名。葛楚天教授多年来从事水生动物遗传与发育研究，在龟鳖类性别决定机制、性控育种等方面取得创新性研究进展，创建了首个龟鳖动物在体基因功能研究技术，鉴定一系列龟鳖性别决定和分化的关键基因及其调控级联，原创性揭示一种龟性别决定的表观遗传调控机制，提出了“温度-表观遗传-性别决定基因”假说，研发中华鳖雄性控制和单性育苗技术，并实现产业化应用。葛楚天教授现任浙江万里学院生物与环境学院院长、动物性别与发育重点研究所所长、宁波市重点实验室（A类）主任，是国家优秀青年科学基金获得者、浙江省高校高水平创新团队带头人、浙江省杰出青年科学基金获得者、浙江省高校创新领军人才、宁波市有突出贡献专家。葛楚天教授曾于2018年、2020年在国际顶尖学术期刊 Science 上发表两篇研究论文，这是宁波地区、浙江省属高校，以及全国水产领域首次发表 Science 论文，属于历史性突破。性别决定模式一般分为基因型性别决定（genetic sex determination，GSD）和环境依赖型性别决定（environmental sex determination，ESD）两大类。而温度依赖型性别决定（temperature-dependent sex determination，TSD）是ESD中最典型的性别决定模式，其最主要特点是缺少性染色体、胚胎孵化的温度决定子代性别，该模式常见于爬行动物（例如红耳龟）。红耳龟在较低的孵化温度（26℃）产生的子代100%为雄性，而在较高的温度（32℃）则全部为雌性。 2018年5月11日，浙江万里学院葛楚天、钱国英、杜克大学Blanche Capel教授等在 Science 期刊发表了题为：The histone demethylase KDM6B regulates temperature-dependent sex determination in a turtle species 的研究论文，在爬行动物温度依赖型性别决定研究领域取得重要进展。该研究揭示了组蛋白去甲基化酶KDM6B调控红耳龟温度依赖型性别决定的分子机理，为“表观遗传机制调控温度依赖型性别决定”假说提供了首个直接遗传证据，解开了温度依赖型性别决定研究长达半个世纪的谜团。 2020年4月17日，浙江万里学院葛楚天、杜克大学Blanche Capel等人在 Science 期刊发表了题为：Temperature-dependent sex determination is mediated by pSTAT3 repression of Kdm6b 的研究论文。该研究发现高温通过促进龟性腺体细胞Ca2+内流，激活pSTAT3，抑制Kdm6b表达，继而抑制Dmrt1表达，最终促进雌性发育；并提出了一个环境依赖型性别决定（ESD）机制的保守调控假说。该研究阐明了龟的温度依赖型性别决定（TSD）系统的上游调控机制，为全面破译龟TSD机制奠定了重要基础，同时提出了一个新的调控假说—“pSTAT3-Kdm6b保守盒”，为其他众多ESD机制研究指明了方向。