Objective: To study preparation technol. of Lianfan Zhixie dispersible tablets. To establish a method of multi-measurement evaluation for the determination of 3 components in Lianfan Zhixie dispersible tablets (berberine hydrochloride, paeoniflorin, baicalin). Methods: The formulations were optimized with disintegration time, in vitro dissolution rate, dispersion homogeneity and rigidity as reference parameters. HPLC was performed to determine the contents, using Alltima C18 chromatog. column (250 mm×4.6 mm, 5 μm), mobile phase: acetonitrile (A), 0.1% triethylamine -0.4% phosphoric acid (B), gradient elution: 0-15 min, 5%-25% A, 15-30 min, 25%-40% A, 30-45 min, 40%-100% A, and 45-50 min, 100% A, flow rate: 1.0 mL/min, column temperature: 30 °C, detection wavelength: 348 nm. Results: Disintegrating agent was 20% CMS-Na (with an internal and external ratio of 5:1), and adhesive agent was 5% PVPK30 solution The dispersible tablets thus prepared were good. A method for the determination of berberine hydrochloride, paeoniflorin and baicalin was established. Conclusion: The prescription is reasonable, the quality indexes conform to the requirement of dispersible tablets. It can be used for quality control of Lianfan Zhixie dispersible tablets.