Objective: More effective control of related substances of etimicin sulfate and sodium chloride injection. Methods: The new standard YBH01062014 using HPLC-ELSD method determination of related substances, the old standard of WS-807(x-593)-2002 using Pre-column derivatization HPLC-UV method determination of related substances, as compared with the exptl. data, anal. what kind of method can more effectively control the related substances. Results: Through the anal. of the exptl. data, the new standards is more effective control than the old standard on the related substances of etimicin sulfate and sodium chloride injection. Conclusion: The related substance of new standard by etimicin sulfate and sodium chloride injection was more strict control, better control of product quality and ensure clin. efficacy.