Objective: To determine the contents of veratridine after the compatibility of Veratrum Nigrum with Panax ginseng and Panax Notoginseng and Panax quinquefolium.Methods: An RP-HPLC method was used to determine the contents of veratridine after the compatibility of Veratrum Nigrum with Panax ginseng and Panax Notoginseng and Panax quinquefolium.The anal. column was Agilent-TC C18 (250mm × 4.6mm, 5 μm).The mobile phase was acetonitrile - 0.1 mol/L ammonium acetate (35 : 65), with the flow rate of 1.0mL · min-1 and detected at 220 nm.Results: The content of veratridine was higher after the compatibility of Panax ginseng and Panax notoginseng and Panax quinquefolium.Conclusion: The method is simple, accurate and sensitive.The article offered an exptl. base for eighteen incompatible pairs.