AbstractObjective: To analyze the interference of HbE on the HbA1c results on detection systems manufactured by Shenzhen Lifotronic. Methods: Volunteers were recruited from Zhongshan city, Guangdong province during June 2020 to November 2020. A total of 55 specimens were obtained, including 40 cases without HbE(23 cases in normal group and 17 cases in diabetes group) and 15 cases with HbE variant. HbA1c of these two groups (normal and HbE group) were analyzed by Shenzhen Lifotronic H8, H9 and GH-900Plus, Bio-Rad Variant II Turbo 2.0 and D-10 from USA, Arkray HA-8180 from Japan, statistical analysis was conducted on the difference between detection results of normal group and HbE variant group. Bio-Rad Variant II Turbo 2.0 was used as the comparison system to calculate the bias and to evaluate the interference of HbE on each system. Results: In normal group, the results of each detection system were comparable to those of Variant II Turbo 2.0, with a bias of -6%~6%. In HbE variant group,system bias of H8, H9, GH-900Plus and D-10 was within -6% and 6%, and the results showed no significant difference (P>0.05), while the negative bias of HA-8180 showed significant difference (P<0.05). Conclusion Compared with Bio-Rad Variant II Turbo 2.0, HbA1c can be correctly detected by H8, H9 and GH-900Plus system without the interference of HbE.