Objective: To evaluate the application of the independent innovation reagent in the automatic determination of 5'-nucleotidasc (5'-NT) activity in the clin. serum or plasma samples.Methods: 5'-NT activities were determined with Olympus AU680 automatic biochem. analyzer by repeatability and linearity tests, the evaluation of methodol. was carried out and the results were compared with those determined by the reagent of Diazyme LaboratoriesResults: The coefficients of variation (CV) of the enzyme activity at two levels of quality control were 2.83% and 1.96%.The determination of enzyme activity showed a good linearity over the range of 5-200 U/L.There was a good correlation between two reagents (r = 0.995).Conclusion: The 5'-nuclcotidasc reagent made by our company can provide a simple, quick, accurate and stable method for the determination of 5'-NT activity in the clin. serum or plasma samples and meet the requirement of the automated anal. of mass clin. samples.This method may be worthy of promotion.