作者: Zhao, Luyang ; Deng, Liwei ; Li, Gailing ; Jin, Huan ; Cai, Jinsen ; Shang, Huan ; Li, Yan ; Yang, Andrew X. ; Chen, Fang ; Zhao, Zhi ; Xu, Guanjie ; Liu, Wuxing ; Liu, Siyu ; Xiang, Guobing ; Liu, Bin ; Xu, Weibin ; Zeng, Lidong ; Zhang, Renli ; Zhao, Huan ; Wu, Ping ; Zhou, Zhiliang ; Zheng, Jiao ; Ezanno, Pierre ; Chen, Weiyue ; Yan, Qin ; Deem, Michael W. ; Yu, Jun ; He, Jiankui
A review. Next generation sequencing (NGS) has revolutionized life sciences research. Recently, a new class of third-generation sequencing platforms has arrived to meet increasing demands in the clinic, capable of directly measuring DNA and RNA sequences at the single-mol. level without amplification. Here, we use the new GenoCare single mol. sequencing platform from Direct Genomics to resequence the E. coli genome and show comparable performance to the Illumina MiSeq system. Our platform detects single-mol. fluorescence by total internal reflection microscopy, with sequencing-by-synthesis chem. With a consensus sequence of 99.71% nucleotide identity to that of the Illumina MiSeq system's, GenoCare was determined to be a reliable platform for single-mol. sequencing, with strong potential for clin. applications.