The HPLC fingerprints of 11 batches of Engelhardia roxburghiana Wall leaves from different regions of Guangxi province were studied for their quality control. The HPLC separation was achieved on a reverse phase DiamonsilR C18 (2) column (4.6 mm × 250 mm, 5 μm) with a C18 guard column (4.6 mm × 15 mm, 5 μm) maintained at 30°C. The mobile phase was acetonitrile-water containing 0.05% phosphoric acid (19 : 81). The flow rate was set at 1.0 mL · min-1. The detection wavelength was 240 nm. The injection volume was 10 μL. The analyzing time was 60 min. Similarity evaluation system for chromatog. fingerprint of TCM published by Chinese Pharmacopoeia Commission (Version: 2004A) was used in data anal. A sensitive, reproducible and selective detection method and a rapid, stable and simple extraction procedure were developed and validated. A reference fingerprint spectrum was established and 11 co-possessing peaks were selected as fingerprints. The similarity between the reference fingerprint spectrum and each fingerprint spectrum from the eleven samples was analyzed and all the similarity values were ≥0.95. The method for fingerprint spectrum establishment is scientific and reasonable and can be used for quality control of Engelhardia roxburghiana leaves from origination in Guangxi.