The presence of trace antibiotics in water can lead to the development of drug-resistant bacterial strains, posing risks to ecosystems and human health. Immunomagnetic separation (IMS) using magnetic nanoparticles (MNPs) is an effective technique for targeted enrichment. This study established and optimized a separation system for the immunomagnetic microsphere enrichment of enrofloxacin (ENR) antibiotics, achieving efficient enrichment and isolation of ENR. To address potential elution degradation, an analysis of ENR degradation pathways and toxicity assessment of degradation products was performed. The study manifested the successful conjugation of antibodies to magnetic microspheres, leading to a 97.68% separation efficiency for ENR in water through IMS. Specifically, 1 mg of MNP@Ab could specifically bind to 1.5 ng of ENR at 37 °C for 30 min, and the elution rate exceeded 83%. No degradation products of ENR were detected during the enrichment and isolation procedures. Nevertheless, extending the elution time to 1 h disclosed three major degradation pathways with higher toxicity risks than ENR based on ecological risk assessment. To strictly control the elution temperature and elution time, the increase in temperature and time will heighten the risk of degradation products. This study presents an efficient strategy for water treatment and environmental protection.

2024-10-01·WATER AIR AND SOIL POLLUTION

Degradation of Diclofenac in Water Using S2O82−/Fe2O3-zeolite Catalyst Synergistic with Ozone

作者: Wang, Xiaoxia ; Shen, Zheng ; Wang, Jiaqi ; Song, Yuanbo ; Jin, Mengyu ; Hou, Cheng ; Xu, Qingsong ; Zhang, Yalei ; Bi, Dongsu ; Wang, Zhenqi

A number of water pollution issues caused by antibiotic effluent must be addressed immediately.The persulfate (PS) method is a sort of advanced oxidation process that has the advantages of a quick reaction time and a high oxidation capacity.However, in order to be used efficiently for wastewater treatment, PS must be activated in water.In this study, PS was directly loaded onto the precursor to produce an oxidant that could destroy organic contaminants in water synergistically with O₃.The effects of oxidant production circumstances, oxidant dosage, O₃ flow rate, PS dose, and pH value on diclofenac (DCF) degradation efficiency were investigated.The results showed that when the initial concentration of DCF was 100 mg/L, the O₃ flow rate was 0.4 m3/h, the pH value was 3, and the oxidant dosage was 1 g/L, the S₂O₈^2-/Fe₂O₃-zeolite/O3 system basically completely degraded DCF within 60 min.The EPR and free radical quenching experiments were used to explore the synergistic degradation of DCF by the S₂O₈^2-/Fe₂O₃-zeolite/O3 system.The three possible degradation pathways of DCF were also proposed based on anal. intermediate products anal. with LC-MS.This study identifies potential DCF breakdown pathways and offers a cost-effective and practical multiphase catalyst for the treatment of organic wastewater.

2023-10-16·Angewandte Chemie (International ed. in English)

Histone Deacetylase 3-Directed PROTACs Have Anti-inflammatory Potential by Blocking Polarization of M0-like into M1-like Macrophages.

Article

作者: Río-Bergé, Clàudia ; Dekker, Frank J ; Zhang, Jianqiu ; Van Der Wouden, Petra E ; Daemen, Toos ; Zhao, Chunlong ; Chen, Deng ; Chen, Shipeng

Macrophage polarization plays a crucial role in inflammatory processes. The histone deacetylase 3 (HDAC3) has a deacetylase-independent function that can activate pro-inflammatory gene expression in lipopolysaccharide-stimulated M1-like macrophages and cannot be blocked by traditional small-molecule HDAC3 inhibitors. Here we employed the proteolysis targeting chimera (PROTAC) technology to target the deacetylase-independent function of HDAC3. We developed a potent and selective HDAC3-directed PROTAC, P7, which induces nearly complete HDAC3 degradation at low micromolar concentrations in both THP-1 cells and human primary macrophages. P7 increases the anti-inflammatory cytokine secretion in THP-1-derived M1-like macrophages. Importantly, P7 decreases the secretion of pro-inflammatory cytokines in M1-like macrophages derived from human primary macrophages. This can be explained by the observed inhibition of macrophage polarization from M0-like into M1-like macrophage. In conclusion, we demonstrate that the HDAC3-directed PROTAC P7 has anti-inflammatory activity and blocks macrophage polarization, demonstrating that this molecular mechanism can be targeted with small molecule therapeutics.

100 项与 P-7 相关的药物交易

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