Myco-Nanofabrication of Silver Nanoparticles by Penicillium brasilianum NP5 and Their Antimicrobial, Photoprotective and Anticancer Effect on MDA-MB-231 Breast Cancer Cell Line.

Article

作者: Kumar, Raju Suresh ; Gunagambhire, Pooja Vidyasagar ; Perumal, Karthikeyan ; Rudrappa, Muthuraj ; Nagaraja, Shashiraj Kareyellappa ; Hiremath, Halaswamy ; Almansour, Abdulrahman I. ; Nayaka, Sreenivasa

Currently, the exploration of fungal organisms for novel metabolite production and its pharmacological applications is much appreciated in the biomedical field. In the present study, the fungal strains were isolated from soil of unexplored Yellapura regions. The potent isolate NP5 was selected based on preliminary screening and identified as Penicillium brasilianum NP5 through morphological, microscopic, and molecular characterizations. Synthesis of silver nanoparticles from P. brasilianum was confirmed by the color change of the reaction mixture and UV-visible surface plasmon resonance (SPR) spectra of 420 nm. Fourier transform infrared (FTIR) analysis revealed the functional groups involved in synthesis. Atomic force microscopy (AFM) and transmission electron microscope (TEM) analysis showed aggregation of the NPs, with sizes ranged from 10 to 60 nm, an average particle size of 25.32 nm, and a polydispersity index (PDI) of 0.40. The crystalline nature and silver as the major element in NP5-AgNPs was confirmed by X-ray diffraction (XRD) and energy dispersive X-ray (EDX) analysis. The negative value -15.3 mV in Zeta potential exhibited good stability, and thermostability was recorded by thermogravimetric analysis (TGA). NP5-AgNPs showed good antimicrobial activity on selected human pathogens in a concentration-dependent manner. The MTT assay showed concentration-dependent anticancer activity with an IC₅₀ of 41.93 µg/mL on the MDA-MB-231 cell line. Further, apoptotic study was carried out by flow cytometry to observe the rate of apoptosis. The calculated sun protection factor (SPF) value confirms good photoprotection capacity. From the results obtained, NP5-AgNPs can be used in the pharmaceutical field after successful in vitro clinical studies.

2013-04-01·Journal of colloid and interface science2区 · 化学

Influence of surfactant polydispersity on the structure of polyoxyethylene (5) nonylphenyl ether/cyclohexane/water reverse microemulsions

2区 · 化学

Article

作者: Sergey A. Tovstun ; Anastasia V. Ivanchikhina ; Vladimir F. Razumov

The distribution of the number of oxyethylene groups in molecules of polyoxyethylene (5) nonylphenyl ether (NP5) is shown to significantly affect the structure of NP5/cyclohexane/water reverse microemulsions. The effect is caused by the difference in solubility of NP5 components. This difference leads to fractionation: surfactant molecules dissolved in the intermicellar nonpolar medium have, on average, shorter polyoxyethylene chains than surfactant molecules forming reverse micelles. A model is proposed which takes into account the influence of this fractionation on reverse micelle size and free surfactant concentration. The validity of this model for NP5/cyclohexane/water reverse microemulsions is experimentally confirmed by dynamic light scattering. It is found that changing the degree of surfactant fractionation in the above mentioned microemulsions can result in the formation of non-spherical reverse micelles.

2012-07-01·Microbiology (Reading, England)4区 · 生物学

Two identical nonylphenol monooxygenase genes linked to IS6100 and some putative insertion sequence elements in Sphingomonas sp. NP5

4区 · 生物学

Article

作者: Nishiyama, Naoki ; Kato, Dai-ichiro ; Negoro, Seiji ; Takeo, Masahiro ; Kitamura, Chitoshi ; Maeda, Yoshihiro ; Maeda, Junko

Sphingomonas sp. NP5 can degrade a wide range of nonylphenol (NP) isomers that have widely contaminated aquatic environments as major endocrine-disrupting chemicals. To understand the biochemical and genetic backgrounds of NP degradation, a gene library of strain NP5 was constructed using a broad-host-range vector pBBR1MCS-2 and introduced into Sphingobium japonicum UT26. Several transformants accumulated reddish brown metabolites on agar plates dispersed with a mixture of NP isomers. Two different DNA fragments (7.6 and 9.3 kb) involved in the phenotype were isolated from the transformants. Sequence analysis revealed that both fragments contained an identical 1593 bp monooxygenase gene (nmoA), the predicted protein sequence of which showed 83 % identity to the octylphenol-4-monooxygenase of Sphingomonas sp. PWE1. The nmoA gene in the 7.6 kb fragment was surrounded by an IS21-type insertion sequence (IS) and IS6100, while another in the 9.3 kb fragment was adjacent to an IS66-type IS, suggesting that they have been acquired through multiple transposition events. A fast-growing recombinant Pseudomonas putida strain harbouring nmoA was constructed and used for degradation of a chemically synthesized NP isomer, 4-(1-ethyl-1-methylhexyl)phenol. This strain converted the isomer into hydroquinone stoichiometrically. 3-Methyl-3-octanol, probably originating from the alkyl side chain, was also detected as the metabolite. These results indicate that these two nmoA genes are involved in the NP degradation ability of strain NP5.

100 项与 NP-5 相关的药物交易

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