ETHNOPHARMACOLOGICAL RELEVANCEAlcoholic Liver Disease (ALD), a chronic condition caused by long-term heavy alcohol consumption, can progress to cirrhosis or liver failure. HuGan Tablets (HGT) is a compound preparation made of six Chinese herbs, which is used in clinic for the treatment of chronic hepatitis, with studies demonstrating its efficacy in alleviating alcohol-induced liver injury in rats. However, the active components and therapeutic targets of HGT remain unclear and require further investigation.AIM OF THIS STUDYThe aim of this study was to develop a systematic pipeline based on the SPR fishing strategy to identify effective components and therapeutic targets in Chinese formulas, using HGT as a representative case.MATERIALS AND METHODSHRMS was employed to analyze HGT ingredients absorbed in rat blood, while network pharmacology, molecular docking and literature mining were utilized to identify potential targets of HGT for ALD alleviation. A systematic SPR-based fishing system was developed by evaluating protein target coupling efficiency, sample recovery rate, specificity of target-small molecule binding, and LOD, and candidate components screened and identified using this system were further screened by SPR affinity tests. Additionally, therapeutic efficacy of the selected compounds was validated in vitro using an ethanol-induced AML12 model and further confirmed in vivo using a mouse model of ALD by assessing markers such as ALT, AST, and oxidative stress indicators.RESULTSA total of 128 compounds were identified in HGT, with 29 metabolites detected in rat blood. MFN2, SOD2, mTOR, RXRA, and GSTP1 were identified as anti-ALD targets of HGT through integrated network pharmacology, molecular docking, and literature analysis. An SPR-based active component fishing system was successfully developed, capturing 15 candidate compounds. SPR affinity analysis revealed strong binding (K_D: 3.41-221.7 μM) between (R,S)-goitrin, chlorogenic acid, saikosaponin B2, schisandrin, schisandrol B, schisandrin A, schisandrin C, and schisantherin A and the target proteins. Except for (R,S)-goitrin, the other seven compounds significantly reduced ALT, AST, TG, ROS, and MDA levels while enhancing SOD and GSH activities in cellular models, with comparable therapeutic effects observed in ALD mice.CONCLUSIONThis study scientifically established an integrated SPR-based pipeline to systematically characterize active ingredients and therapeutic targets in herbal formulations, which was successfully applied to reveal key therapeutic targets and pharmacodynamic components of HGT for ALD. This study provides a valuable framework for SPR-based screening of bioactive components in traditional formulas, as well as for understanding the material basis and mechanism of action of HGT in the treatment of ALD.

2025-03-01·Industrial Crops and Products

Optimization of somatic embryogenesis system and accumulation of secondary metabolites in callus of Schisandra chinensis (Turcz.) Baill

作者: Liu, Jiu-Shi ; Zhang, Xuan-He ; Sun, Dan ; Shi, Guang-Li ; Li, Xue-Ting ; Wang, Zhen-Xing ; Liang, Long-Jun ; Ai, Jun ; Li, Meng

Schisandra chinensis (Turcz.) Baill is a widely recognized herb in traditional Chinese medicine, valued for its hepatoprotective, anti-tumor, and anti-HIV properties, as well as its diverse range of bioactive compoundsThis study aimed to optimize the somatic embryogenesis system of S. chinensis, overcome spatiotemporal limitations, and explore the medicinal potential of S. chinensis callus as a plant-based medicine.The results indicated that subterranean stems achieved a 100% callus induction rate under 25° C and 5000 Lx light intensity, while dormant buds exhibited the highest embryogenic callus induction rate at 34.44%.Significant differences in morphol. and histol. were observed between non-embryogenic and embryogenic calluses.Among the five genotypes examined, the ′Zaohong′ genotype demonstrated the highest capacity for inducing callus and embryogenic callus.The addition of exogenous nutrients markedly enhanced the proliferation rate of embryogenic callus, with the highest proliferation rate (262.33%) observed when 500 mg / L inositol was supplemented.Furthermore, coconut juice was identified as the most effective additive for somatic embryo induction and synchronized development, outperforming banana and potato juices.Plant growth regulators, light and temperature significantly influenced callus proliferation and secondary metabolite accumulation.The optimal combination for callus proliferation and metabolite synthesis was 3 mg/L NAA and 0.2 mg/L TDZ.Addnl., 5000 Lx light intensity significantly boosted secondary metabolite production compared to 2500 Lx or darkness.Cultivation at 25 ° C was beneficial for callus proliferation and lignan synthesis, whereas 18 ° C optimized the accumulation of lignans and gomisins.This study enhanced the regeneration efficiency of the somatic embryogenesis system in S.chinensis, and identified an effective culture protocol for high yields of lignans and gomisins, providing a foundation for industrial-scale production and biotechnol. development of its active compounds

2025-03-01·Journal of Food Composition and Analysis

Highly efficient extraction and analysis of Schisandra chinensis lignans via magnetic multivariate metal-organic framework with a porous polymer coating

作者: Wu, Yongning ; Dang, Yike ; Yang, Guojuan ; Wang, Qinyuan ; Li, Wenrui ; Li, Guoliang ; Wang, Ruihong ; Li, Fan

Lignans from Schisandra chinensis have strong anti-hepatotoxic, antioxidant, and anti-tumor properties.Herein, a multivariate metal-organic framework (MTV-MOF) was synthesized with biphenyl dicarboxylic acid, 3,5-diaminobenzoic acid (DABA), and aluminum-based metal salts, introduced 1,8-dihydroxynaphthalene (AM) as a porous coating, constructed a new magnetic material (Fe₃O₄@DUT-5-DABA@AM) and utilized in magnetic solid-phase extraction (MSPE) as potent adsorbents to adsorb Schizandrol A, Schizandrol B, Schisantherin A, Schisandrin A, and Schizandrin B, five lignans.Prepared materials were characterized using FT-IR, XRD, TEM, SEM, VSM, zeta and nitrogen adsorption-desorption analyses.At the same time, their adsorption kinetics and isothermal adsorption lines were also evaluated.Due to electrostatic interactions, π stacking interactions, and hydrogen bonding, the composite exhibited excellent extraction capability for lignans.In conjunction with HPLC-MS, the MSPE approach demonstrated promising linearity (R² ≥ 0.9992) and low detection limits (0.024-0.2 μg·kg^-1) for lignans under optimal circumstances.The approach efficaciously quantified lignans in S. chinensis extract, attaining recoveries of 93.4-116.6 % with relative standard deviations ≤ 6.3 %.Compared with traditional methods, the magnetic separation method proposed in this study is simple, economical, and practical with low solvent consumption, which has unique advantages in the anal. of natural active ingredients.

100 项与 Besigomsin 相关的药物交易

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