[Complete genomic sequence of rapidly replicating strain MB-7 of hepatitis a virus and its characterization in comparison with nucleotide sequences of other hepatitis A virus strains].

Article

作者: Nemtsov, Iu V ; Ternovoĭ, V A ; Iashin, V V ; Nikulin, L G ; Kusliĭ, A G ; Netesov, S V ; Kriuk, N I ; Chausov, E V ; Muntianova, M A ; Bondarenko, T Iu ; Sviatchenko, V A ; Kiselev, N N

Complete nucleotide sequence of genomic RNA of hepatitis A virus (HAV) rapidly replicating strain MB-7 was determined. Comparison of nucleotide and deduced amino-acid sequences demonstrated the highest level of identity of MB-7 with strain HAS-15 (above 99%) and high homology with other HAV strains (HM 175/7, CR326, and GBM/HFS) used in production of anti-hepatitis A vaccines. MB-7 was classified as subgenotype IA. Phylogenetic analysis showed that MB-7 is most closely related to the strain HAS-15 and the HAV variants circulating in Russia. Comparative analysis of genomic differences between MB-7 and HAS-15 with other HAV strains revealed among changes characteristic of MB-7 those typical of the described earlier rapidly replicating HAV strains (nt. 149-162 in 5'-untranslated region and changes in the VP3 and 2C genes). These results suggest the functional importance of changes in above-mentioned regions of HAV genome for the increased replication level of MB-7 in vitro.

Voprosy virusologii

[The isolation and study of the characteristics of a cytopathic strain of the hepatitis A virus].

Article

作者: Kalashnikova, T V ; Karpovich, L G ; Sosnovtsev, S V ; Nemtsov, Iu V ; Chizhikov, V E ; Tiunnikov, G I ; Maĭdaniuk, A G ; Konakova, V E ; Mamaeva, N V ; Bondarenko, E P

A fast-growing cytopathic isolate of human hepatitis A virus (strain MB-7) was derived from fecal samples of infected patients and adapted to growth in FRhK-4 cell culture. A positive serum standard against HAV and electron microscopy were used to demonstrate that MB-7 belonged to human hepatitis A virus. The strain MB-7 induced plaque formation in FRhK-4 under agar overlay after 10-12 days of incubation. The PCR products of gene VP1 were cloned in E. coli and its primary structure was determined. MB-7 was shown to have more homology with HAV strains isolated in the USA and China.

100 项与 MB-7 相关的药物交易

登录后查看更多信息