K-Ras based vaccine(ASCENDO Biotechnology)

An industrial method is presented through a model with a chamber for aerogenic immunization of about 250 pigs, aged 2.5 months. The experiments were carried out with more than 1,000 pigs of the Camborough hybrid under industrial conditions of raising. The process of immunization was feasibly carried out when the pigs passed through the chamber using aerosol at mixing them upon transference from the growing section to the fattening one, according to the provisions of the raising technology. A lapinized K-vaccine against fever, obtained from rabbits or tissue cultures was used in a dispersed state. Essential proved the high titer of the vaccine. Used were the aerosol devices DAG-2 or Microjet Fogmaster mounted at 1.5 m above the chamber floor. They guarantee the production of highly dispersed aerosol of an average arithmetic size of 7-10 microns, its concentration reaching 3,000,000 particles per 1 cm3. The animals were exposed for 30 min with Fogmaster and 45 min with DAG-2. It was shown that they tolerated perfectly this group immunization. A single procedure with the vaccine conferred immunity at 2.10(5) LT50 and protected the pigs from fever over the entire fattening period. The method was evaluated as a biotechnology.

A phage specific for Escherichia coli K5 antigen was used to determine the frequency of K5 in strains of E. coli isolated from cases of sepsis, meningitis, and urinary tract infection (pyelonephritis, cystitis, and asymptomatic bacteriuria), as well as from fecal samples of healthy subjects. Although entirely absent from meningitic strains, K5 was found to be one of the most common E. coli capsular antigens, especially in strains causing sepsis. K5 was less common in the fecal strains of healthy subjects than in strains causing various urinary tract infections, between which there was no significant difference in its frequency. Thus, even if K5 is a less important antigen than K1 in the etiology of acute pyelonephritis or acute meningitis, as the fifth most commonly occurring K antigen in E. coli strains causing urinary tract infection it should be included as a component in any K antigen-based vaccine against acute pyelonephritis.