Abscisic Acid

Algae extracts are utilized as biofertilizers instead of chemical ferti-lizers in agriculture. Further, algae are known to possess a high content of plant hormones, such as gibberellin, salicylic acid, abscisic acid, and brassinosteroids.

The main objective of this study was to increase the extraction yield and simulta-neously extract hormones required for plant growth from Sargassum swartzii using Magnetic recoverable ionic liquid (IL).

In this study, extraction was performed by acidic digestion with acetic acid and then alkaline digestion with potassium hydroxide.

The results showed the ionic liquid effect in extraction yield by 266 percent. The extracted phytohormones were analyzed using high-performance liquid chromatography (HPLC) methods. High levels of gibberellin, salicylic acid, abscisic acid, and brassinosteroids in improved algae extraction showed that seaweed extract could be used as environmentally friendly liquid bio-fertilizers to replace chemical fertilizers and could play a crucial role in organic farming for sustainable agriculture. Additionally, the recoverability of ionic liquid eight times with negligible leaching proved the introduced procedure to be cost-effective.

The reported procedure for algae extraction improved by using an acidic/primary ionic liquid environment. This procedure is economical because of the simple reusability of ionic liquid due to its magnetic features.

The reported procedure for algae extraction improved by using an acidic/primary ionic liquid environment. This procedure is economic because of the simply reusability of ionic liquid due to its magnetic features.

Sodium nitrophenate (CSN) enhanced cold tolerance of cucumber. However, at the omics-level, the molecular mechanism of CSN to cold stress remains unclear. Here, we found that CSN was comparable to abscisic acid and much stronger than 2, 4-epibrassinolide (EBR) in enhancing cold tolerance. RNA-seq indicated that CSN regulated the brassinolides (BR) and cytokinin (CK) synthesis in the late stage of cold stress (LS-CS). CSN reduced the source of BR synthesis, accelerated the conversion of intermediate substances to BR and the deactivation of BR. While, CSN accelerated CK synthesis and CK deactivation by cytokinin dehydrogenase. Hormone content determination showed that CSN increased BR and decreased CK contents during most time-points of cold stress. Kinds of hormone signaling genes at LS-CS were activated by CSN, which may be due to changes in BR and CK contents. CSN also enhanced the expression of 90 % phenylalanine ammonia-lyase genes, participated in phenylpropanoid biosynthesis, at LS-CS. Genes of phenylpropanoid biosynthesis pathway and hormones signal were co-expression during cold stress. The metabolome also showed that CSN participated phenylpropanoid biosynthesis at LS-CS too. However, as for lipid metabolome, CSN up-regulated anthocyanin, flavones and flavonols metabolism at the early stage of cold stress. The autumn and winter field yield test showed that CSN increase cucumber yield by approximately 17.67 % and economic income by 207.67 dollars/667 m². Collectedly, CSN may regulate lipid metabolism and hormone signaling mediated antioxidant pathways to enhance cold tolerance in the early and late stages of cold stress, respectively.